Detection of total parenteral nutrition-induced fatty liver infiltration in the rat by in vitro proton nuclear magnetic resonance

D. O. Jacobs, R. G. Settle, S. O. Trerotola, J. E. Albina, G. L. Wolf, J. L. Rombeau

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In an effort to determine if NMR techniques might be used to detect TPN-induced hepatic steatosis, the NMR spin-lattice (T1) and spin-spin (T2) relaxation times were measured on liver tissue from rats who received one of five dietary regimens: (1) 100% of nonprotein calories as lipid (Fat); (2) a mixture of 50% lipid and 50% glucose nonprotein calories (50/50); (3) 100% of nonprotein calories as glucose (CHO); (4) intravenous saline and standard laboratory rat chow (Saline); and (5) rat chow alone (Oral). The parenteral diets were isonitrogenous and isocaloric. Serum liver function tests were also measured. Animals in the Fat and 50/50 groups had the greatest amounts of liver fat and significantly longer T1 and T2 times (p < 0.01) than any other group. Furthermore, the correlation of T2 time with liver fat content (r = 0.82) was far superior (p < 0.001) to that of serum SGPT (r = 0.48) which was the only liver function test which correlated significantly with liver fat content. In a multiple linear regression analysis, T1 and T2 predicted liver fat content with an r value of 0.84 (p < 0.001). These data suggest that in vivo NMR imaging techniques might be used to detect TPN-induced fatty infiltration of the liver noninvasively.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalJournal of Parenteral and Enteral Nutrition
Volume10
Issue number2
StatePublished - 1986
Externally publishedYes

Fingerprint

Total Parenteral Nutrition
total parenteral nutrition
fatty liver
Fatty Liver
protons
Protons
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Fats
liver
Liver
rats
Liver Function Tests
liver function
lipid content
lipids
Lipids
Glucose
glucose
animal fats and oils

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Jacobs, D. O., Settle, R. G., Trerotola, S. O., Albina, J. E., Wolf, G. L., & Rombeau, J. L. (1986). Detection of total parenteral nutrition-induced fatty liver infiltration in the rat by in vitro proton nuclear magnetic resonance. Journal of Parenteral and Enteral Nutrition, 10(2), 177-183.

Detection of total parenteral nutrition-induced fatty liver infiltration in the rat by in vitro proton nuclear magnetic resonance. / Jacobs, D. O.; Settle, R. G.; Trerotola, S. O.; Albina, J. E.; Wolf, G. L.; Rombeau, J. L.

In: Journal of Parenteral and Enteral Nutrition, Vol. 10, No. 2, 1986, p. 177-183.

Research output: Contribution to journalArticle

Jacobs, D. O. ; Settle, R. G. ; Trerotola, S. O. ; Albina, J. E. ; Wolf, G. L. ; Rombeau, J. L. / Detection of total parenteral nutrition-induced fatty liver infiltration in the rat by in vitro proton nuclear magnetic resonance. In: Journal of Parenteral and Enteral Nutrition. 1986 ; Vol. 10, No. 2. pp. 177-183.
@article{ff224aee1f2e41878cf6c7c0f6e71472,
title = "Detection of total parenteral nutrition-induced fatty liver infiltration in the rat by in vitro proton nuclear magnetic resonance",
abstract = "In an effort to determine if NMR techniques might be used to detect TPN-induced hepatic steatosis, the NMR spin-lattice (T1) and spin-spin (T2) relaxation times were measured on liver tissue from rats who received one of five dietary regimens: (1) 100{\%} of nonprotein calories as lipid (Fat); (2) a mixture of 50{\%} lipid and 50{\%} glucose nonprotein calories (50/50); (3) 100{\%} of nonprotein calories as glucose (CHO); (4) intravenous saline and standard laboratory rat chow (Saline); and (5) rat chow alone (Oral). The parenteral diets were isonitrogenous and isocaloric. Serum liver function tests were also measured. Animals in the Fat and 50/50 groups had the greatest amounts of liver fat and significantly longer T1 and T2 times (p < 0.01) than any other group. Furthermore, the correlation of T2 time with liver fat content (r = 0.82) was far superior (p < 0.001) to that of serum SGPT (r = 0.48) which was the only liver function test which correlated significantly with liver fat content. In a multiple linear regression analysis, T1 and T2 predicted liver fat content with an r value of 0.84 (p < 0.001). These data suggest that in vivo NMR imaging techniques might be used to detect TPN-induced fatty infiltration of the liver noninvasively.",
author = "Jacobs, {D. O.} and Settle, {R. G.} and Trerotola, {S. O.} and Albina, {J. E.} and Wolf, {G. L.} and Rombeau, {J. L.}",
year = "1986",
language = "English (US)",
volume = "10",
pages = "177--183",
journal = "JPEN. Journal of parenteral and enteral nutrition",
issn = "0148-6071",
publisher = "SAGE Publications Inc.",
number = "2",

}

TY - JOUR

T1 - Detection of total parenteral nutrition-induced fatty liver infiltration in the rat by in vitro proton nuclear magnetic resonance

AU - Jacobs, D. O.

AU - Settle, R. G.

AU - Trerotola, S. O.

AU - Albina, J. E.

AU - Wolf, G. L.

AU - Rombeau, J. L.

PY - 1986

Y1 - 1986

N2 - In an effort to determine if NMR techniques might be used to detect TPN-induced hepatic steatosis, the NMR spin-lattice (T1) and spin-spin (T2) relaxation times were measured on liver tissue from rats who received one of five dietary regimens: (1) 100% of nonprotein calories as lipid (Fat); (2) a mixture of 50% lipid and 50% glucose nonprotein calories (50/50); (3) 100% of nonprotein calories as glucose (CHO); (4) intravenous saline and standard laboratory rat chow (Saline); and (5) rat chow alone (Oral). The parenteral diets were isonitrogenous and isocaloric. Serum liver function tests were also measured. Animals in the Fat and 50/50 groups had the greatest amounts of liver fat and significantly longer T1 and T2 times (p < 0.01) than any other group. Furthermore, the correlation of T2 time with liver fat content (r = 0.82) was far superior (p < 0.001) to that of serum SGPT (r = 0.48) which was the only liver function test which correlated significantly with liver fat content. In a multiple linear regression analysis, T1 and T2 predicted liver fat content with an r value of 0.84 (p < 0.001). These data suggest that in vivo NMR imaging techniques might be used to detect TPN-induced fatty infiltration of the liver noninvasively.

AB - In an effort to determine if NMR techniques might be used to detect TPN-induced hepatic steatosis, the NMR spin-lattice (T1) and spin-spin (T2) relaxation times were measured on liver tissue from rats who received one of five dietary regimens: (1) 100% of nonprotein calories as lipid (Fat); (2) a mixture of 50% lipid and 50% glucose nonprotein calories (50/50); (3) 100% of nonprotein calories as glucose (CHO); (4) intravenous saline and standard laboratory rat chow (Saline); and (5) rat chow alone (Oral). The parenteral diets were isonitrogenous and isocaloric. Serum liver function tests were also measured. Animals in the Fat and 50/50 groups had the greatest amounts of liver fat and significantly longer T1 and T2 times (p < 0.01) than any other group. Furthermore, the correlation of T2 time with liver fat content (r = 0.82) was far superior (p < 0.001) to that of serum SGPT (r = 0.48) which was the only liver function test which correlated significantly with liver fat content. In a multiple linear regression analysis, T1 and T2 predicted liver fat content with an r value of 0.84 (p < 0.001). These data suggest that in vivo NMR imaging techniques might be used to detect TPN-induced fatty infiltration of the liver noninvasively.

UR - http://www.scopus.com/inward/record.url?scp=0022625344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022625344&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 177

EP - 183

JO - JPEN. Journal of parenteral and enteral nutrition

JF - JPEN. Journal of parenteral and enteral nutrition

SN - 0148-6071

IS - 2

ER -