Detection of trichothecene mycotoxins in sera from individuals exposed to stachybotrys chartarum in indoor environments

Trevor L. Brasel, Andrew W. Campbell, Roger E. Demers, Bruce S. Ferguson, Jordan Fink, Aristo Vojdani, Stephen C. Wilson, David C. Straus

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To date, no study has effectively demonstrated a direct human exposure to mycotoxins in mold-contaminated buildings. Therefore, the authors investigated the presence of trichothecene mycotoxins in sera from individuals exposed to indoor molds (specifically Stachybotrys chartarum). Sera from occupants of contaminated (test samples, n = 44) and uncontaminated (control samples, n = 26) buildings were analyzed using a competitive enzyme-linked immunosorbent assay (ELISA) highly specific for macrocyclic trichothecenes. Twenty-three samples were significantly different (p < 0.05) from normal human serum tested in the same manner, whereas only 1 of the control samples tested positive. Mass spectrometry analysis could not confirm the presence of intact S. chartarum macrocyclic trichothecenes. The authors hypothesize that this result was caused by uncharacterized ELISA-reactive metabolic breakdown products. Data from this study suggest that trichothecene mycotoxins can be demonstrated in the tissues of certain individuals exposed to S. chartarum in contaminated buildings.

Original languageEnglish (US)
Pages (from-to)317-323
Number of pages7
JournalArchives of Environmental Health
Volume59
Issue number6
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Stachybotrys
Trichothecenes
Mycotoxins
serum
Immunosorbents
Serum
assay
enzyme
Assays
Fungi
Enzyme-Linked Immunosorbent Assay
Molds
Enzymes
mass spectrometry
Mass spectrometry
Mass Spectrometry
Tissue
indoor environment
detection

Keywords

  • Environmental exposure
  • Mold
  • Mycotoxins
  • Serum
  • Stachybotrys chartarum

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Brasel, T. L., Campbell, A. W., Demers, R. E., Ferguson, B. S., Fink, J., Vojdani, A., ... Straus, D. C. (2004). Detection of trichothecene mycotoxins in sera from individuals exposed to stachybotrys chartarum in indoor environments. Archives of Environmental Health, 59(6), 317-323.

Detection of trichothecene mycotoxins in sera from individuals exposed to stachybotrys chartarum in indoor environments. / Brasel, Trevor L.; Campbell, Andrew W.; Demers, Roger E.; Ferguson, Bruce S.; Fink, Jordan; Vojdani, Aristo; Wilson, Stephen C.; Straus, David C.

In: Archives of Environmental Health, Vol. 59, No. 6, 06.2004, p. 317-323.

Research output: Contribution to journalArticle

Brasel, TL, Campbell, AW, Demers, RE, Ferguson, BS, Fink, J, Vojdani, A, Wilson, SC & Straus, DC 2004, 'Detection of trichothecene mycotoxins in sera from individuals exposed to stachybotrys chartarum in indoor environments', Archives of Environmental Health, vol. 59, no. 6, pp. 317-323.
Brasel, Trevor L. ; Campbell, Andrew W. ; Demers, Roger E. ; Ferguson, Bruce S. ; Fink, Jordan ; Vojdani, Aristo ; Wilson, Stephen C. ; Straus, David C. / Detection of trichothecene mycotoxins in sera from individuals exposed to stachybotrys chartarum in indoor environments. In: Archives of Environmental Health. 2004 ; Vol. 59, No. 6. pp. 317-323.
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