Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon

Erika Oliveira Gomes; Lívia Sacchetto; Maurício Teixeira; Bárbara Aparecida Chaves; Adam Hendy; Claudia Mendonça; Izabele Guimarães; Ramon Linhares; Daniela Brito; Danielle Valério; Jady Shayenne Mota Cordeiro; Alexandre Vilhena Silva Neto; Vanderson Souza Sampaio; Vera Margarete Scarpassa; Michaela Buenemann; Nikos Vasilakis; Djane Clarys Baia-da-Silva; Maurício Lacerda Nogueira; Maria Paula Gomes Mourão; Marcus Vinícius Guimarães Lacerda

doi:10.3390/v15061356

Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon

Erika Oliveira Gomes, Lívia Sacchetto, Maurício Teixeira, Bárbara Aparecida Chaves, Adam Hendy, Claudia Mendonça, Izabele Guimarães, Ramon Linhares, Daniela Brito, Danielle Valério, Jady Shayenne Mota Cordeiro, Alexandre Vilhena Silva Neto, Vanderson Souza Sampaio, Vera Margarete Scarpassa, Michaela Buenemann, Nikos Vasilakis, Djane Clarys Baia-da-Silva, Maurício Lacerda Nogueira, Maria Paula Gomes Mourão, Marcus Vinícius Guimarães Lacerda

Pathology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Zika virus (ZIKV) is an RNA flavivirus (Flaviviridae family) endemic in tropical and subtropical regions that is transmitted to humans by Aedes (Stegomyia) species mosquitoes. The two main urban vectors of ZIKV are Aedes aegypti and Aedes albopictus, which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female Ae. aegypti (22 specimens) and Ae. albopictus (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) Ae. aegypti and 5/241 (2%) Ae. albopictus pools screened using RT-qPCR were positive for ZIKV. No supernatants from Ae. aegypti were positive for ZIKV (0%), and 15 out of 241 (6.2%) Ae. albopictus pools were positive. In this study, we provide the first-ever evidence of Ae. albopictus naturally infected with ZIKV in the Amazon region.

Original language	English (US)
Article number	1356
Journal	Viruses
Volume	15
Issue number	6
DOIs	https://doi.org/10.3390/v15061356
State	Published - Jun 2023

Keywords

Amazon
ZIKV
arbovirus
forest
mosquito surveillance

ASJC Scopus subject areas

Infectious Diseases
Virology

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10.3390/v15061356

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Gomes, E. O., Sacchetto, L., Teixeira, M., Chaves, B. A., Hendy, A., Mendonça, C., Guimarães, I., Linhares, R., Brito, D., Valério, D., Cordeiro, J. S. M., Neto, A. V. S., Sampaio, V. S., Scarpassa, V. M., Buenemann, M., Vasilakis, N., Baia-da-Silva, D. C., Nogueira, M. L., Mourão, M. P. G., & Lacerda, M. V. G. (2023). Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon. Viruses, 15(6), Article 1356. https://doi.org/10.3390/v15061356

Gomes, EO, Sacchetto, L, Teixeira, M, Chaves, BA, Hendy, A, Mendonça, C, Guimarães, I, Linhares, R, Brito, D, Valério, D, Cordeiro, JSM, Neto, AVS, Sampaio, VS, Scarpassa, VM, Buenemann, M, Vasilakis, N, Baia-da-Silva, DC, Nogueira, ML, Mourão, MPG & Lacerda, MVG 2023, 'Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon', Viruses, vol. 15, no. 6, 1356. https://doi.org/10.3390/v15061356

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title = "Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon",

abstract = "Zika virus (ZIKV) is an RNA flavivirus (Flaviviridae family) endemic in tropical and subtropical regions that is transmitted to humans by Aedes (Stegomyia) species mosquitoes. The two main urban vectors of ZIKV are Aedes aegypti and Aedes albopictus, which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female Ae. aegypti (22 specimens) and Ae. albopictus (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) Ae. aegypti and 5/241 (2%) Ae. albopictus pools screened using RT-qPCR were positive for ZIKV. No supernatants from Ae. aegypti were positive for ZIKV (0%), and 15 out of 241 (6.2%) Ae. albopictus pools were positive. In this study, we provide the first-ever evidence of Ae. albopictus naturally infected with ZIKV in the Amazon region.",

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doi = "10.3390/v15061356",

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T1 - Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon

AU - Gomes, Erika Oliveira

AU - Sacchetto, Lívia

AU - Teixeira, Maurício

AU - Chaves, Bárbara Aparecida

AU - Hendy, Adam

AU - Mendonça, Claudia

AU - Guimarães, Izabele

AU - Linhares, Ramon

AU - Brito, Daniela

AU - Valério, Danielle

AU - Cordeiro, Jady Shayenne Mota

AU - Neto, Alexandre Vilhena Silva

AU - Sampaio, Vanderson Souza

AU - Scarpassa, Vera Margarete

AU - Buenemann, Michaela

AU - Vasilakis, Nikos

AU - Baia-da-Silva, Djane Clarys

AU - Nogueira, Maurício Lacerda

AU - Mourão, Maria Paula Gomes

AU - Lacerda, Marcus Vinícius Guimarães

PY - 2023/6

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N2 - Zika virus (ZIKV) is an RNA flavivirus (Flaviviridae family) endemic in tropical and subtropical regions that is transmitted to humans by Aedes (Stegomyia) species mosquitoes. The two main urban vectors of ZIKV are Aedes aegypti and Aedes albopictus, which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female Ae. aegypti (22 specimens) and Ae. albopictus (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) Ae. aegypti and 5/241 (2%) Ae. albopictus pools screened using RT-qPCR were positive for ZIKV. No supernatants from Ae. aegypti were positive for ZIKV (0%), and 15 out of 241 (6.2%) Ae. albopictus pools were positive. In this study, we provide the first-ever evidence of Ae. albopictus naturally infected with ZIKV in the Amazon region.

AB - Zika virus (ZIKV) is an RNA flavivirus (Flaviviridae family) endemic in tropical and subtropical regions that is transmitted to humans by Aedes (Stegomyia) species mosquitoes. The two main urban vectors of ZIKV are Aedes aegypti and Aedes albopictus, which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female Ae. aegypti (22 specimens) and Ae. albopictus (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) Ae. aegypti and 5/241 (2%) Ae. albopictus pools screened using RT-qPCR were positive for ZIKV. No supernatants from Ae. aegypti were positive for ZIKV (0%), and 15 out of 241 (6.2%) Ae. albopictus pools were positive. In this study, we provide the first-ever evidence of Ae. albopictus naturally infected with ZIKV in the Amazon region.

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Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon

Abstract

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