Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes

Dana L. Vanlandingham, Konstantin Tsetsarkin, Kimberly A. Klingler, Chao Hong, Kate L. McElroy, Michael J. Lehane, Stephen Higgs

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The alphaviruses o'nyong nyong virus (ONNV) and chikungunya virus (CHIKV) provide a unique system to study the viral genes involved in vector specificity. ONNV infects both anopheline and culicine mosquitoes, whereas CHIKV infects only culicine mosquitoes. In this study, chimeric viruses were constructed that contained genes from both ONNV and CHIKV. These chimeras and previously described full-length infectious clones of ONNV and CHIKV were evaluated in Anopheles gambiae and Aedes aegypti mosquitoes. Virus derived from the infectious clones of ONNV and CHIKV retained the vector specificity of the parental viruses. All six of the chimeras were found to infect Ae. aegypti mosquitoes at high rates but only the chimera containing viral genes encoding all of the structural proteins of ONNV was able to infect An. gambiae mosquitoes. These data indicate that all of the viral structural proteins are necessary for ONNV to infect An. gambiae mosquitoes.

Original languageEnglish (US)
Pages (from-to)663-669
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume74
Issue number4
StatePublished - Apr 1 2006

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Chikungunya virus
Anopheles
Aedes
Culicidae
Viruses
Anopheles gambiae
Viral Genes
Clone Cells
Alphavirus
Viral Structural Proteins

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Vanlandingham, D. L., Tsetsarkin, K., Klingler, K. A., Hong, C., McElroy, K. L., Lehane, M. J., & Higgs, S. (2006). Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes. American Journal of Tropical Medicine and Hygiene, 74(4), 663-669.

Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes. / Vanlandingham, Dana L.; Tsetsarkin, Konstantin; Klingler, Kimberly A.; Hong, Chao; McElroy, Kate L.; Lehane, Michael J.; Higgs, Stephen.

In: American Journal of Tropical Medicine and Hygiene, Vol. 74, No. 4, 01.04.2006, p. 663-669.

Research output: Contribution to journalArticle

Vanlandingham, DL, Tsetsarkin, K, Klingler, KA, Hong, C, McElroy, KL, Lehane, MJ & Higgs, S 2006, 'Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes', American Journal of Tropical Medicine and Hygiene, vol. 74, no. 4, pp. 663-669.
Vanlandingham DL, Tsetsarkin K, Klingler KA, Hong C, McElroy KL, Lehane MJ et al. Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes. American Journal of Tropical Medicine and Hygiene. 2006 Apr 1;74(4):663-669.
Vanlandingham, Dana L. ; Tsetsarkin, Konstantin ; Klingler, Kimberly A. ; Hong, Chao ; McElroy, Kate L. ; Lehane, Michael J. ; Higgs, Stephen. / Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes. In: American Journal of Tropical Medicine and Hygiene. 2006 ; Vol. 74, No. 4. pp. 663-669.
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