Determination of genome size and restriction fragment length polymorphism of four Chinese rickettsial isolates by pulsed-field gel electrophoresis

J. Z. Zhang, M. Y. Fan

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Pulsed-field gel electrophoresis (PFGE) was used to determine the genome size and the restriction fragment length polymorphism (RFLP) of four new Chinese isolates of spotted fever group (SFG) rickettsiae. The genome size of the isolates Ha-91, BJ-90, 054 and 036 was 1253 kb, 1236 kb, 1272 kb, and 1272 kb, respectively. The isolates 054 and 036 had identical RFLP profiles. All the isolates differed in the properties under study from the so far known SFG rickettsiae. The unique RFLP profiles of the isolates supported our opinion that they are new strains of SFG rickettsiae or even new species of SFG rickettsiae.

Original languageEnglish (US)
Pages (from-to)25-30
Number of pages6
JournalActa Virologica
Volume46
Issue number1
StatePublished - Jan 1 2002

Keywords

  • Chinese isolates
  • Pulsed-field gel electrophoresis
  • Rickettsiae
  • Spotted fever group

ASJC Scopus subject areas

  • Immunology
  • Virology

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