Determination of hydrogen peroxide and peroxynitrite-induced modifications to rabbit skeletal troponin C by online LC ESI FT-ICR MS

Silvia M. Pulido, Mark Emmett, Fei He, Alan G. Marshall

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The determination of hydrogen peroxide and peroxynitrite-induced modifications to rabbit skeletal troponin C by online liquid chromatography electrospray Fourier transform ion cyclotron resonance mass spectrometry (LC ESI FT-ICR MS) was discussed. Muscular weakness resulted from modifications to EF- hand proteins in the contractile apparatus of muscle fibers. Methionine residues 157 and 158 were susceptible to oxidation and contributed to loss of function in TnC. Protein modifications were mapped to specific residues by comparing tryptic digests of treated vs. control TnC. It was found that the data was analyzed with MIDAS software.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages331-332
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Troponin C
Peroxynitrous Acid
Hydrogen Peroxide
Cyclotron resonance
Liquid chromatography
Methionine
Mass spectrometry
Muscle
Fourier transforms
Proteins
Ions
Oxidation
Fibers

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Pulido, S. M., Emmett, M., He, F., & Marshall, A. G. (2002). Determination of hydrogen peroxide and peroxynitrite-induced modifications to rabbit skeletal troponin C by online LC ESI FT-ICR MS. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 331-332)

Determination of hydrogen peroxide and peroxynitrite-induced modifications to rabbit skeletal troponin C by online LC ESI FT-ICR MS. / Pulido, Silvia M.; Emmett, Mark; He, Fei; Marshall, Alan G.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 331-332.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pulido, SM, Emmett, M, He, F & Marshall, AG 2002, Determination of hydrogen peroxide and peroxynitrite-induced modifications to rabbit skeletal troponin C by online LC ESI FT-ICR MS. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 331-332, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Pulido SM, Emmett M, He F, Marshall AG. Determination of hydrogen peroxide and peroxynitrite-induced modifications to rabbit skeletal troponin C by online LC ESI FT-ICR MS. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 331-332
Pulido, Silvia M. ; Emmett, Mark ; He, Fei ; Marshall, Alan G. / Determination of hydrogen peroxide and peroxynitrite-induced modifications to rabbit skeletal troponin C by online LC ESI FT-ICR MS. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 331-332
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