Determination of post-translational modifications of proteins by high-sensitivity, high-resolution Fourier transform ion cyclotron resonance mass spectrometry

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The response of a cell to its extracellular environment is a multi-step process beginning with signal transduction that is governed by "subtle changes" often resulting in protein expression. Proteomics is the tracking of this protein expression. Post-translational modification (PTM) is a "subtle change" that has a major influence on signal transduction. Phosphorylation and glycosylation propagate signals by sequential, reversible modifications. High-sensitivity, high-resolution and multiple MS capabilities of Fourier transform ion cyclotron resonance mass spectrometry permit localization of the PTM(s) with electron-capture dissociation, and then structural determination of the PTM with infrared multiphoton dissociation.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalJournal of Chromatography A
Volume1013
Issue number1-2
DOIs
StatePublished - Sep 26 2003
Externally publishedYes

Keywords

  • Electron-capture dissociation
  • Glycoproteins
  • Infrared multiphoton dissociation
  • Ion cyclotron resonance
  • Lectins
  • Mass spectrometry
  • Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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