Determination of post-translational modifications of proteins by high-sensitivity, high-resolution Fourier transform ion cyclotron resonance mass spectrometry

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16 Citations (Scopus)

Abstract

The response of a cell to its extracellular environment is a multi-step process beginning with signal transduction that is governed by "subtle changes" often resulting in protein expression. Proteomics is the tracking of this protein expression. Post-translational modification (PTM) is a "subtle change" that has a major influence on signal transduction. Phosphorylation and glycosylation propagate signals by sequential, reversible modifications. High-sensitivity, high-resolution and multiple MS capabilities of Fourier transform ion cyclotron resonance mass spectrometry permit localization of the PTM(s) with electron-capture dissociation, and then structural determination of the PTM with infrared multiphoton dissociation.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalJournal of Chromatography A
Volume1013
Issue number1-2
DOIs
StatePublished - Sep 26 2003
Externally publishedYes

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Cyclotrons
Cyclotron resonance
Signal transduction
Fourier Analysis
Post Translational Protein Processing
Mass spectrometry
Mass Spectrometry
Fourier transforms
Ions
Glycosylation
Phosphorylation
Signal Transduction
Proteins
Infrared radiation
Proteomics
Electrons

Keywords

  • Electron-capture dissociation
  • Glycoproteins
  • Infrared multiphoton dissociation
  • Ion cyclotron resonance
  • Lectins
  • Mass spectrometry
  • Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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AB - The response of a cell to its extracellular environment is a multi-step process beginning with signal transduction that is governed by "subtle changes" often resulting in protein expression. Proteomics is the tracking of this protein expression. Post-translational modification (PTM) is a "subtle change" that has a major influence on signal transduction. Phosphorylation and glycosylation propagate signals by sequential, reversible modifications. High-sensitivity, high-resolution and multiple MS capabilities of Fourier transform ion cyclotron resonance mass spectrometry permit localization of the PTM(s) with electron-capture dissociation, and then structural determination of the PTM with infrared multiphoton dissociation.

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KW - Glycoproteins

KW - Infrared multiphoton dissociation

KW - Ion cyclotron resonance

KW - Lectins

KW - Mass spectrometry

KW - Proteins

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