Determining Persistence of Bocavirus DNA in the Respiratory Tract of Children by Pyrosequencing

Johana Castro Wagner, Richard Pyles, Aaron L. Miller, J. Nokso-Koivisto, Michael J. Loeffelholz, Tasnee Chonmaitree

Research output: Contribution to journalArticle

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Abstract

BACKGROUND:: Although human bocavirus type 1 (HBoV1) is a respiratory pathogen, presence of HBoV-DNA in secretions of asymptomatic children raised the question on the significance of HBoV-positive results. METHODS:: Archived specimens from a prospective, longitudinal study were tested for HBoV. A total of 94 children (aged 6-36 m) were HBoV(+) during 172 upper respiratory tract infection (URI) and/ or acute otitis media (AOM) episodes. We used pyrosequencing of NP1, VP1 and VP2 genes to type HBoV and subtype HBoV1 in these specimens. RESULTS:: Of the specimens tested, HBoV-DNA were successfully sequenced in 128 (74%) samples from 70 children; all were HBoV type 1. Subtypes identified (n=108) were: LWK/TW (63%), LWK/BJ (20%), Bonn/BJ (16%) and LWK/KU3 (1%). Of 46 children for whom shedding pattern could be determined, viral clearance within 30d (13-29d) occurred in 28%; another 22% of children had no recurrence after 32 to 267d. Prolonged virus presence of >30 d (34 to 181d+) occurred in 22%; intermittent detection (61+ to 170d+) in 20%. Infection with the same HBoV1 subtype after 4-5 negative samples (244 and 265d interval) occurred in 4%. Infection with 2 different HBoV1 subtypes (29 and 87d apart) occurred in only 4%. Newly acquired HBoV1-URI resulted in AOM in 53% of cases. CONCLUSIONS:: Children with HBoV1 infection commonly shed for a prolonged period leading to repeated viral DNA detection. Recurrence after 8-9 m suggests possible persistence and reactivation. Infections with 2 different HBoV1 subtypes within one-year period are uncommon. Newly acquired HBoV1-URI is often complicated by AOM.

Original languageEnglish (US)
JournalPediatric Infectious Disease Journal
DOIs
StateAccepted/In press - Jan 13 2016

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Bocavirus
Human bocavirus
Respiratory System
DNA
Otitis Media
Respiratory Tract Infections
Infection
Recurrence
Viral DNA
Longitudinal Studies
Prospective Studies
Viruses

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Determining Persistence of Bocavirus DNA in the Respiratory Tract of Children by Pyrosequencing. / Castro Wagner, Johana; Pyles, Richard; Miller, Aaron L.; Nokso-Koivisto, J.; Loeffelholz, Michael J.; Chonmaitree, Tasnee.

In: Pediatric Infectious Disease Journal, 13.01.2016.

Research output: Contribution to journalArticle

Castro Wagner, Johana ; Pyles, Richard ; Miller, Aaron L. ; Nokso-Koivisto, J. ; Loeffelholz, Michael J. ; Chonmaitree, Tasnee. / Determining Persistence of Bocavirus DNA in the Respiratory Tract of Children by Pyrosequencing. In: Pediatric Infectious Disease Journal. 2016.
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abstract = "BACKGROUND:: Although human bocavirus type 1 (HBoV1) is a respiratory pathogen, presence of HBoV-DNA in secretions of asymptomatic children raised the question on the significance of HBoV-positive results. METHODS:: Archived specimens from a prospective, longitudinal study were tested for HBoV. A total of 94 children (aged 6-36 m) were HBoV(+) during 172 upper respiratory tract infection (URI) and/ or acute otitis media (AOM) episodes. We used pyrosequencing of NP1, VP1 and VP2 genes to type HBoV and subtype HBoV1 in these specimens. RESULTS:: Of the specimens tested, HBoV-DNA were successfully sequenced in 128 (74{\%}) samples from 70 children; all were HBoV type 1. Subtypes identified (n=108) were: LWK/TW (63{\%}), LWK/BJ (20{\%}), Bonn/BJ (16{\%}) and LWK/KU3 (1{\%}). Of 46 children for whom shedding pattern could be determined, viral clearance within 30d (13-29d) occurred in 28{\%}; another 22{\%} of children had no recurrence after 32 to 267d. Prolonged virus presence of >30 d (34 to 181d+) occurred in 22{\%}; intermittent detection (61+ to 170d+) in 20{\%}. Infection with the same HBoV1 subtype after 4-5 negative samples (244 and 265d interval) occurred in 4{\%}. Infection with 2 different HBoV1 subtypes (29 and 87d apart) occurred in only 4{\%}. Newly acquired HBoV1-URI resulted in AOM in 53{\%} of cases. CONCLUSIONS:: Children with HBoV1 infection commonly shed for a prolonged period leading to repeated viral DNA detection. Recurrence after 8-9 m suggests possible persistence and reactivation. Infections with 2 different HBoV1 subtypes within one-year period are uncommon. Newly acquired HBoV1-URI is often complicated by AOM.",
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AU - Loeffelholz, Michael J.

AU - Chonmaitree, Tasnee

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N2 - BACKGROUND:: Although human bocavirus type 1 (HBoV1) is a respiratory pathogen, presence of HBoV-DNA in secretions of asymptomatic children raised the question on the significance of HBoV-positive results. METHODS:: Archived specimens from a prospective, longitudinal study were tested for HBoV. A total of 94 children (aged 6-36 m) were HBoV(+) during 172 upper respiratory tract infection (URI) and/ or acute otitis media (AOM) episodes. We used pyrosequencing of NP1, VP1 and VP2 genes to type HBoV and subtype HBoV1 in these specimens. RESULTS:: Of the specimens tested, HBoV-DNA were successfully sequenced in 128 (74%) samples from 70 children; all were HBoV type 1. Subtypes identified (n=108) were: LWK/TW (63%), LWK/BJ (20%), Bonn/BJ (16%) and LWK/KU3 (1%). Of 46 children for whom shedding pattern could be determined, viral clearance within 30d (13-29d) occurred in 28%; another 22% of children had no recurrence after 32 to 267d. Prolonged virus presence of >30 d (34 to 181d+) occurred in 22%; intermittent detection (61+ to 170d+) in 20%. Infection with the same HBoV1 subtype after 4-5 negative samples (244 and 265d interval) occurred in 4%. Infection with 2 different HBoV1 subtypes (29 and 87d apart) occurred in only 4%. Newly acquired HBoV1-URI resulted in AOM in 53% of cases. CONCLUSIONS:: Children with HBoV1 infection commonly shed for a prolonged period leading to repeated viral DNA detection. Recurrence after 8-9 m suggests possible persistence and reactivation. Infections with 2 different HBoV1 subtypes within one-year period are uncommon. Newly acquired HBoV1-URI is often complicated by AOM.

AB - BACKGROUND:: Although human bocavirus type 1 (HBoV1) is a respiratory pathogen, presence of HBoV-DNA in secretions of asymptomatic children raised the question on the significance of HBoV-positive results. METHODS:: Archived specimens from a prospective, longitudinal study were tested for HBoV. A total of 94 children (aged 6-36 m) were HBoV(+) during 172 upper respiratory tract infection (URI) and/ or acute otitis media (AOM) episodes. We used pyrosequencing of NP1, VP1 and VP2 genes to type HBoV and subtype HBoV1 in these specimens. RESULTS:: Of the specimens tested, HBoV-DNA were successfully sequenced in 128 (74%) samples from 70 children; all were HBoV type 1. Subtypes identified (n=108) were: LWK/TW (63%), LWK/BJ (20%), Bonn/BJ (16%) and LWK/KU3 (1%). Of 46 children for whom shedding pattern could be determined, viral clearance within 30d (13-29d) occurred in 28%; another 22% of children had no recurrence after 32 to 267d. Prolonged virus presence of >30 d (34 to 181d+) occurred in 22%; intermittent detection (61+ to 170d+) in 20%. Infection with the same HBoV1 subtype after 4-5 negative samples (244 and 265d interval) occurred in 4%. Infection with 2 different HBoV1 subtypes (29 and 87d apart) occurred in only 4%. Newly acquired HBoV1-URI resulted in AOM in 53% of cases. CONCLUSIONS:: Children with HBoV1 infection commonly shed for a prolonged period leading to repeated viral DNA detection. Recurrence after 8-9 m suggests possible persistence and reactivation. Infections with 2 different HBoV1 subtypes within one-year period are uncommon. Newly acquired HBoV1-URI is often complicated by AOM.

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