Detoxification of xenobiotics by glutathione S-transferases in erythrocytes: The transport of the conjugate of glutathione and 1-chloro-2,4-dinitrobenzene

Y. C. Awasthi, G. Misra, D. K. Rassin

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Abstract

The incubation of human erythrocytes with 1-chloro-2,4-dinitrobenzene (CDNB) results in almost quantitative conjugation of glutathione (GSH) to form S-(2,4-dinitrophenyl) glutathione. The reaction is catalysed by erythrocyte glutathione S-transferase. During the present studies we have identified the conjugate in the incubation medium of CDNB-treated erythrocytes, indicating that the conjugate of GSH and CDNB is transported out by the erythrocytes. Quantitation of the conjugate in the incubation medium by amino acid analysis and thin layer chromatography indicates that the erythrocytes transport the conjugate at an approximate rate of 140 nmol/h/ml erythrocytes. The transport of the conjugate is inhibited by sodium fluoride. Exhaustion of ATP from the erythrocytes results in a significant decrease in the rate of transport which is restored with the regeneration of ATP by incubating the erythrocytes with adenine and inosine. This indicates that the transport of conjugate is an energy dependent process.

Original languageEnglish (US)
Pages (from-to)419-425
Number of pages7
JournalBritish Journal of Haematology
Volume55
Issue number3
StatePublished - 1983

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Xenobiotics
Glutathione Transferase
Erythrocytes
Adenosine Triphosphate
Dinitrochlorobenzene
Sodium Fluoride
Inosine
1-chloro-2,4-dinitrobenzene-glutathione conjugate
Adenine
Thin Layer Chromatography
Glutathione
Regeneration
Amino Acids

ASJC Scopus subject areas

  • Hematology

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Detoxification of xenobiotics by glutathione S-transferases in erythrocytes : The transport of the conjugate of glutathione and 1-chloro-2,4-dinitrobenzene. / Awasthi, Y. C.; Misra, G.; Rassin, D. K.

In: British Journal of Haematology, Vol. 55, No. 3, 1983, p. 419-425.

Research output: Contribution to journalArticle

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AU - Rassin, D. K.

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AB - The incubation of human erythrocytes with 1-chloro-2,4-dinitrobenzene (CDNB) results in almost quantitative conjugation of glutathione (GSH) to form S-(2,4-dinitrophenyl) glutathione. The reaction is catalysed by erythrocyte glutathione S-transferase. During the present studies we have identified the conjugate in the incubation medium of CDNB-treated erythrocytes, indicating that the conjugate of GSH and CDNB is transported out by the erythrocytes. Quantitation of the conjugate in the incubation medium by amino acid analysis and thin layer chromatography indicates that the erythrocytes transport the conjugate at an approximate rate of 140 nmol/h/ml erythrocytes. The transport of the conjugate is inhibited by sodium fluoride. Exhaustion of ATP from the erythrocytes results in a significant decrease in the rate of transport which is restored with the regeneration of ATP by incubating the erythrocytes with adenine and inosine. This indicates that the transport of conjugate is an energy dependent process.

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