Detoxification of xenobiotics by glutathione S‐transferases in erythrocytes: the transport of the conjugate of glutathione and 1‐chloro‐2,4‐dinitrobenzene

Yogesh C. Awasthi, Gopal Misra, David K. Rassin, Satish Srivastava

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Summary. The incubation of human erythrocytes with 1‐chloro‐2,4‐dinitrobenzene (CDNB) results in almost quantitative conjugation of glutathione (GSH) to form S‐(2,4‐dinitrophenyl) glutathione. The reaction is catalysed by erythrocyte glutathione S‐transferase. During the present studies we have identified the conjugate in the incubation medium of CDNB‐treated erythrocytes, indicating that the conjugate of GSH and CDNB is transported out by the erythrocytes. Quantitation of the conjugate in the incubation medium by amino acid analysis and thin layer chromatography indicates that the erythrocytes transport the conjugate at an approximate rate of 140 nmol/h/ml erythrocytes. The transport of the conjugate is inhibited by sodium fluoride. Exhaustion of ATP from the erythrocytes results in a significant decrease in the rate of transport which is restored with the regeneration of ATP by incubating the erythrocytes with adenine and inosine. This indicates that the transport of conjugate is an energy dependent process.

Original languageEnglish (US)
Pages (from-to)419-425
Number of pages7
JournalBritish Journal of Haematology
Volume55
Issue number3
DOIs
StatePublished - Nov 1983

ASJC Scopus subject areas

  • Hematology

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