Developing a new interdisciplinary lab course for undergraduate and graduate students

Plant cells and proteins

Joseph M. Jez, Daniel P. Schachtman, R. Howard Berg, Christopher G. Taylor, Sixue Chen, Leslie M. Hicks, Jan G. Jaworski, Thomas Smith, Erik Nielsen, Craig S. Pikaard

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Studies of protein function increasingly use multifaceted approaches that span disciplines including recombinant DNA technology, cell biology, and analytical biochemistry. These studies rely on sophisticated equipment and methodologies including confocal fluorescence microscopy, mass spectrometry, and X-ray crystallography that are beyond the scope of traditional laboratory courses. To equip the advanced undergraduate and beginning graduate students with an enabling base of knowledge and initial experience with advanced protein research methodologies, a laboratory course entitled Plant Cells and Proteins was developed in a partnership between Washington University and the Donald Danforth Plant Science Center in St. Louis. In this one semester course, 10-12 students obtain hands-on experience with plant tissue culture, gene transformation, subcellular localization of fluorescent recombinant proteins using confocal microscopy, purification of affinity-tagged recombinant proteins, isolation of total protein extracts, enzymatic assays, one- and two-dimensional gel electrophoresis, MALDI-TOF and ESI-Q-TOF mass spectrometry, protein crystallization, and X-ray diffraction. The course is taught as a series of modules, each led by an expert researcher. Students are evaluated based on a series of graded written reports and tests of their mastery of key concepts, interpretations, and the limitations of the experimental methods.

Original languageEnglish (US)
Pages (from-to)410-415
Number of pages6
JournalBiochemistry and Molecular Biology Education
Volume35
Issue number6
DOIs
StatePublished - Nov 2007
Externally publishedYes

Fingerprint

Plant Proteins
Plant Cells
Students
Recombinant Proteins
Confocal Microscopy
Confocal microscopy
Mass Spectrometry
Proteins
Mass spectrometry
Knowledge Bases
Recombinant DNA
Cytology
X Ray Crystallography
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Enzyme Assays
Genetic engineering
Crystallization
Tissue culture
Fluorescence Microscopy

Keywords

  • Fundamental skills
  • Graduate
  • Interdisciplinary
  • Teaching laboratory
  • Undergraduate

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Jez, J. M., Schachtman, D. P., Berg, R. H., Taylor, C. G., Chen, S., Hicks, L. M., ... Pikaard, C. S. (2007). Developing a new interdisciplinary lab course for undergraduate and graduate students: Plant cells and proteins. Biochemistry and Molecular Biology Education, 35(6), 410-415. https://doi.org/10.1002/bmb.99

Developing a new interdisciplinary lab course for undergraduate and graduate students : Plant cells and proteins. / Jez, Joseph M.; Schachtman, Daniel P.; Berg, R. Howard; Taylor, Christopher G.; Chen, Sixue; Hicks, Leslie M.; Jaworski, Jan G.; Smith, Thomas; Nielsen, Erik; Pikaard, Craig S.

In: Biochemistry and Molecular Biology Education, Vol. 35, No. 6, 11.2007, p. 410-415.

Research output: Contribution to journalArticle

Jez, JM, Schachtman, DP, Berg, RH, Taylor, CG, Chen, S, Hicks, LM, Jaworski, JG, Smith, T, Nielsen, E & Pikaard, CS 2007, 'Developing a new interdisciplinary lab course for undergraduate and graduate students: Plant cells and proteins', Biochemistry and Molecular Biology Education, vol. 35, no. 6, pp. 410-415. https://doi.org/10.1002/bmb.99
Jez, Joseph M. ; Schachtman, Daniel P. ; Berg, R. Howard ; Taylor, Christopher G. ; Chen, Sixue ; Hicks, Leslie M. ; Jaworski, Jan G. ; Smith, Thomas ; Nielsen, Erik ; Pikaard, Craig S. / Developing a new interdisciplinary lab course for undergraduate and graduate students : Plant cells and proteins. In: Biochemistry and Molecular Biology Education. 2007 ; Vol. 35, No. 6. pp. 410-415.
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