Development and characterization of a guinea pig-adapted Sudan virus

Gary Wong, Shihua He, Haiyan Wei, Andrea Kroeker, Jonathan Audet, Anders Leung, Todd Cutts, Jill Graham, Darwyn Kobasa, Carissa Embury-Hyatt, Gary P. Kobinger, Xiangguo Qiu

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Infections with Sudan virus (SUDV), a member of the genus Ebolavirus, result in a severe hemorrhagic fever with a fatal outcome in over 50% of human cases. The paucity of prophylactics and therapeutics against SUDV is attributed to the lack of a small-animal model to screen promising compounds. By repeatedly passaging SUDV within the livers and spleens of guinea pigs in vivo, a guinea pig-adapted SUDV variant (SUDV-GA) uniformly lethal to these animals, with a 50% lethal dose (LD50) of 5.3×10-2 50% tissue culture infective doses (TCID50), was developed. Animals infected with SUDV-GA developed high viremia and died between 9 and 14 days postinfection. Several hallmarks of SUDV infection, including lymphadenopathy, increased liver enzyme activities, and coagulation abnormalities, were observed. Virological analyses and gross pathology, histopathology, and immunohistochemistry findings indicate that SUDV-GA replicates in the livers and spleens of infected animals similarly to SUDV infections in nonhuman primates. These developments will accelerate the development of specific medical countermeasures in preparation for a future disease outbreak due to SUDV.

Original languageEnglish (US)
Pages (from-to)392-399
Number of pages8
JournalJournal of virology
Volume90
Issue number1
DOIs
StatePublished - 2016
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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