Development and characterization of a stable luciferase dengue virus for high-throughput screening

Gang Zou, Hao Ying Xu, Min Qing, Qing Yin Wang, Pei-Yong Shi

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

To facilitate dengue virus (DENV) drug discovery, we developed a stable luciferase reporter DENV-2. A renilla luciferase gene was engineered into the capsid-coding region of an infectious cDNA clone of DENV-2. Transfection of BHK-21 cells with the cDNA clone-derived RNA generated high titers (>10 6PFU/ml) of luciferase reporter DENV-2. The reporter virus was infectious to a variety of cells, producing robust luciferase signals. Compared with wild-type virus, the reporter virus replicated slower in both mammalian Vero and mosquito C6/36 cells. To examine the stability of the reporter virus, we continuously passaged the virus on Vero cells for five rounds. All passaged viruses stably maintained the luciferase gene, demonstrating the stability of the reporter virus. Furthermore, we found that the passaged virus accumulated a mutation (T108M) in viral NS4B gene that could enhance viral RNA replication in a cell-type specific manner. Using the reporter virus, we developed a HTS assay in a 384-well format. The HTS assay was validated with known DENV inhibitors and showed a robust Z′ factor of 0.79. The Luc-DENV-2 HTS assay allows screening for inhibitors of all steps of the viral life cycle. The reporter virus will also be a useful tool for studying DENV replication and pathogenesis.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalAntiviral Research
Volume91
Issue number1
DOIs
StatePublished - Jul 2011
Externally publishedYes

Fingerprint

Dengue Virus
Luciferases
Viruses
Renilla Luciferases
Complementary DNA
Clone Cells
Vero Cells
Viral Genes
Capsid
Viral RNA
Drug Discovery
Virus Replication
Life Cycle Stages
Culicidae
Genes
Transfection
RNA

Keywords

  • Antiviral drug discovery
  • Dengue virus
  • High-throughput screening
  • Luciferase reporter

ASJC Scopus subject areas

  • Virology
  • Pharmacology

Cite this

Development and characterization of a stable luciferase dengue virus for high-throughput screening. / Zou, Gang; Xu, Hao Ying; Qing, Min; Wang, Qing Yin; Shi, Pei-Yong.

In: Antiviral Research, Vol. 91, No. 1, 07.2011, p. 11-19.

Research output: Contribution to journalArticle

Zou, Gang ; Xu, Hao Ying ; Qing, Min ; Wang, Qing Yin ; Shi, Pei-Yong. / Development and characterization of a stable luciferase dengue virus for high-throughput screening. In: Antiviral Research. 2011 ; Vol. 91, No. 1. pp. 11-19.
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