Development and characterization of West Nile virus replicon expressing secreted Gaussia Luciferase

Chao Shan; Xiaodan Li; Chenglin Deng; Baodi Shang; Linlin Xu; Hanqing Ye; Zhiming Yuan; Bo Zhang

doi:10.1007/s12250-013-3332-7

Development and characterization of West Nile virus replicon expressing secreted Gaussia Luciferase

Chao Shan, Xiaodan Li, Chenglin Deng, Baodi Shang, Linlin Xu, Hanqing Ye, Zhiming Yuan, Bo Zhang

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.

Original language	English (US)
Pages (from-to)	161-166
Number of pages	6
Journal	Virologica Sinica
Volume	28
Issue number	3
DOIs	https://doi.org/10.1007/s12250-013-3332-7
State	Published - Jun 2013
Externally published	Yes

Keywords

Flavivirus
High-throughput assay
Replicon
West Nile virus

ASJC Scopus subject areas

Infectious Diseases
Molecular Medicine
Virology
Immunology

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10.1007/s12250-013-3332-7

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title = "Development and characterization of West Nile virus replicon expressing secreted Gaussia Luciferase",

abstract = "We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.",

keywords = "Flavivirus, High-throughput assay, Replicon, West Nile virus",

author = "Chao Shan and Xiaodan Li and Chenglin Deng and Baodi Shang and Linlin Xu and Hanqing Ye and Zhiming Yuan and Bo Zhang",

note = "Funding Information: We gratefully thank Dr Pei-Yong Shi for assistance during this work. This work was supported by the National Natural Science Foundation of China (grant No. 31170158 and 31000090) and the {\textquoteleft}100 Talents Project{\textquoteright} of Chinese Academy of Sciences, China (grant No. Y002171YC1).",

year = "2013",

month = jun,

doi = "10.1007/s12250-013-3332-7",

language = "English (US)",

volume = "28",

pages = "161--166",

journal = "Virologica Sinica",

issn = "1674-0769",

publisher = "Ko hsueh chu pan she",

number = "3",

}

TY - JOUR

T1 - Development and characterization of West Nile virus replicon expressing secreted Gaussia Luciferase

AU - Shan, Chao

AU - Li, Xiaodan

AU - Deng, Chenglin

AU - Shang, Baodi

AU - Xu, Linlin

AU - Ye, Hanqing

AU - Yuan, Zhiming

AU - Zhang, Bo

N1 - Funding Information: We gratefully thank Dr Pei-Yong Shi for assistance during this work. This work was supported by the National Natural Science Foundation of China (grant No. 31170158 and 31000090) and the ‘100 Talents Project’ of Chinese Academy of Sciences, China (grant No. Y002171YC1).

PY - 2013/6

Y1 - 2013/6

N2 - We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.

AB - We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.

KW - Flavivirus

KW - High-throughput assay

KW - Replicon

KW - West Nile virus

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DO - 10.1007/s12250-013-3332-7

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AN - SCOPUS:84879242817

SN - 1674-0769

VL - 28

SP - 161

EP - 166

JO - Virologica Sinica

JF - Virologica Sinica

IS - 3

ER -

Development and characterization of West Nile virus replicon expressing secreted Gaussia Luciferase

Abstract

Keywords

ASJC Scopus subject areas

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