Development and performance of a multiplex PCR assay for the detection of bacteria in sterile body fluids

Coreen Johnson; Christopher Marquez; Damon Olson; Tabitha Ward; Stephen Cheney; Tina Hulten; Trang Ton; C. R. Webb; James Dunn

doi:10.2217/fmb-2022-0226

Development and performance of a multiplex PCR assay for the detection of bacteria in sterile body fluids

Coreen Johnson, Christopher Marquez, Damon Olson, Tabitha Ward, Stephen Cheney, Tina Hulten, Trang Ton, C. R. Webb, James Dunn

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Aim: To assess the performance characteristics of a lab-developed multiplex PCR assay for the detection of common bacterial pathogens associated with infections in pediatric patients from normally sterile sites, such as cerebrospinal fluid, synovial and pleural fluids. Materials methods: A total of 272 specimens were tested by PCR and traditional culture methods to assess the presence of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, methicillin-sensitive and methicillin-resistant Staphylococcus aureus and Kingella kingae. Results: Compared with culture, the overall positive and negative percentage agreement of the PCR were 95.9% and 74.1%, respectively. Conclusion: This sterile body fluid PCR affords a rapid and sensitive alternative for bacterial detection, allowing for more timely pathogen-directed antimicrobial therapy.

Original language	English (US)
Pages (from-to)	187-195
Number of pages	9
Journal	Future Microbiology
Volume	18
Issue number	3
DOIs	https://doi.org/10.2217/fmb-2022-0226
State	Published - Feb 1 2023
Externally published	Yes

Keywords

Kingella kingae
cerebrospinal fluid
multiplex PCR
osteoarticular infection
pleural fluid
synovial fluid

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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title = "Development and performance of a multiplex PCR assay for the detection of bacteria in sterile body fluids",

abstract = "Aim: To assess the performance characteristics of a lab-developed multiplex PCR assay for the detection of common bacterial pathogens associated with infections in pediatric patients from normally sterile sites, such as cerebrospinal fluid, synovial and pleural fluids. Materials methods: A total of 272 specimens were tested by PCR and traditional culture methods to assess the presence of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, methicillin-sensitive and methicillin-resistant Staphylococcus aureus and Kingella kingae. Results: Compared with culture, the overall positive and negative percentage agreement of the PCR were 95.9% and 74.1%, respectively. Conclusion: This sterile body fluid PCR affords a rapid and sensitive alternative for bacterial detection, allowing for more timely pathogen-directed antimicrobial therapy.",

keywords = "Kingella kingae, cerebrospinal fluid, multiplex PCR, osteoarticular infection, pleural fluid, synovial fluid",

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T1 - Development and performance of a multiplex PCR assay for the detection of bacteria in sterile body fluids

AU - Johnson, Coreen

AU - Marquez, Christopher

AU - Olson, Damon

AU - Ward, Tabitha

AU - Cheney, Stephen

AU - Hulten, Tina

AU - Ton, Trang

AU - Webb, C. R.

AU - Dunn, James

PY - 2023/2/1

Y1 - 2023/2/1

N2 - Aim: To assess the performance characteristics of a lab-developed multiplex PCR assay for the detection of common bacterial pathogens associated with infections in pediatric patients from normally sterile sites, such as cerebrospinal fluid, synovial and pleural fluids. Materials methods: A total of 272 specimens were tested by PCR and traditional culture methods to assess the presence of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, methicillin-sensitive and methicillin-resistant Staphylococcus aureus and Kingella kingae. Results: Compared with culture, the overall positive and negative percentage agreement of the PCR were 95.9% and 74.1%, respectively. Conclusion: This sterile body fluid PCR affords a rapid and sensitive alternative for bacterial detection, allowing for more timely pathogen-directed antimicrobial therapy.

AB - Aim: To assess the performance characteristics of a lab-developed multiplex PCR assay for the detection of common bacterial pathogens associated with infections in pediatric patients from normally sterile sites, such as cerebrospinal fluid, synovial and pleural fluids. Materials methods: A total of 272 specimens were tested by PCR and traditional culture methods to assess the presence of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, methicillin-sensitive and methicillin-resistant Staphylococcus aureus and Kingella kingae. Results: Compared with culture, the overall positive and negative percentage agreement of the PCR were 95.9% and 74.1%, respectively. Conclusion: This sterile body fluid PCR affords a rapid and sensitive alternative for bacterial detection, allowing for more timely pathogen-directed antimicrobial therapy.

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KW - cerebrospinal fluid

KW - multiplex PCR

KW - osteoarticular infection

KW - pleural fluid

KW - synovial fluid

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Development and performance of a multiplex PCR assay for the detection of bacteria in sterile body fluids

Abstract

Keywords

ASJC Scopus subject areas

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