Development of a FACS-based assay for evaluating antiviral potency of compound in dengue infected peripheral blood mononuclear cells

Yilong Fu, Yen Liang Chen, Maxime Herve, Feng Gu, Pei Yong Shi, Francesca Blasco

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Dengue fever is the most important arthropod-transmitted viral disease affecting humans. It is a blood-borne disease characterized by persistent fever and joint pain. In the blood, primary peripheral blood mononuclear cells (PBMCs), in particular monocytes, are the main target of the dengue virus (DENV). These cells are poorly permissive for in vitro dengue virus infection and their infectivity varies from donor to donor. To overcome this barrier, an anti-dengue antibody was used to improve the infectivity of DENV-2 clinical isolates to PBMCs, the monocytic leukemia cell line, THP-1 and the granulocyte cell line, KU812. A higher throughput 96-well-format assay based on a fluorescent-activated cell sorter could potentially be developed to evaluate the antiviral potency of compounds in DENV-infected PBMCs in vitro. The results correlate well with data obtained by a standard plaque assay. Altogether, an assay has been developed that enables evaluation of the antiviral activity of test compounds in a physiologically-relevant cell system (PBMCs). These screening processes are urgently needed for dengue drug discovery.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalJournal of Virological Methods
Volume196
DOIs
StatePublished - Feb 2014
Externally publishedYes

Keywords

  • Dengue
  • FACS
  • Screening

ASJC Scopus subject areas

  • Virology

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