Development of a new technique for recovery of cytokines from inflammatory sites in situ

Rafeul Alam, Tommy C. Sim, Kimberley Hilsmeier, J. Andrew Grant

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We attempted to recover cytokines from nasal mucosal surface following allergen challenge. Repeated lavage of nasal mucosa of seven allergic patients was done, but we failed to detect IL-1β in the lavage samples even in ten-fold concentrated materials. Therefore, we developed a new technique to recover cytokines using filter strips. Small filter strips were placed on nasal turbinates for 10 min at different time points after allergen challenge. The strips were air-dried, and stored. For recovery of cytokines individual strips were washed with small volumes of Hepes buffer containing 0.3% human serum albumin. Eluates were assayed for the presence of IL-1β and GM-CSF using commercially available ELISA. We were able to detect IL-1β and GM-CSF in eluates. Both cytokines were consistently detectable in the late phase allergic reaction peaking at 5 h. Nasal challenge with saline failed to detect any cytokine during the 7 h observation period. In standardization experiments known quantities of IL-1β and GM-CSF were applied to filter strips and the recovery ranged from 67 to 89%. Thus, we developed a simple technique of recovery of cytokines from inflammatory mucosa in situ.

Original languageEnglish (US)
Pages (from-to)25-29
Number of pages5
JournalJournal of Immunological Methods
Volume155
Issue number1
DOIs
StatePublished - Oct 19 1992

Fingerprint

Cytokines
Interleukin-1
Granulocyte-Macrophage Colony-Stimulating Factor
Nose
Therapeutic Irrigation
Allergens
Turbinates
Nasal Mucosa
Serum Albumin
Buffers
Hypersensitivity
Mucous Membrane
Enzyme-Linked Immunosorbent Assay
Air
Observation

Keywords

  • Cytokine
  • Filter strip
  • Late phase allergic reaction
  • Nasal mucosa

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Development of a new technique for recovery of cytokines from inflammatory sites in situ. / Alam, Rafeul; Sim, Tommy C.; Hilsmeier, Kimberley; Andrew Grant, J.

In: Journal of Immunological Methods, Vol. 155, No. 1, 19.10.1992, p. 25-29.

Research output: Contribution to journalArticle

Alam, Rafeul ; Sim, Tommy C. ; Hilsmeier, Kimberley ; Andrew Grant, J. / Development of a new technique for recovery of cytokines from inflammatory sites in situ. In: Journal of Immunological Methods. 1992 ; Vol. 155, No. 1. pp. 25-29.
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