@article{2d6619cf68b3450dadbff3655c8f2aa1,
title = "Development of a Rapid Focus Reduction Neutralization Test Assay for Measuring SARS-CoV-2 Neutralizing Antibodies",
abstract = "SARS-CoV-2 is a recently emerged human coronavirus that has escalated to a pandemic. There are currently no approved vaccines for SARS-CoV-2, which causes severe respiratory illness or death. Defining the antibody response to SARS-CoV-2 will be essential for understanding disease progression, long-term immunity, and vaccine efficacy. Here we describe two methods for evaluating the neutralization capacity of SARS-CoV-2 antibodies. The basic protocol is a focus reduction neutralization test (FRNT), which involves immunostaining infected cells with a chromogen deposit readout. The alternate protocol is a modification of the FRNT that uses an infectious clone−derived SARS-CoV-2 virus expressing a fluorescent reporter. These protocols are adapted for use in a high-throughput setting, and are compatible with large-scale vaccine studies or clinical testing.",
keywords = "SARS-Cov-2, antibody neutralization, high-throughput neutralization assay, neutralizing antibodies, serological assay",
author = "Abigail Vanderheiden and Edara, {Venkata Viswanadh} and Katharine Floyd and Kauffman, {Robert C.} and Grace Mantus and Evan Anderson and Nadine Rouphael and Sri Edupuganti and Shi, {Pei Yong} and Menachery, {Vineet D.} and Jens Wrammert and Suthar, {Mehul S.}",
note = "Funding Information: Thanks to Kathy Stephens and Laila Hussaini for their assistance in recruiting the patients. The research reported in this publication was supported in part by an Emory EVPHA Synergy Fund award (M.S.S. and J.W.), COVID‐Catalyst‐I Funds from the Woodruff Health Sciences Center (M.S.S), Center for Childhood Infections and Vaccines (M.S.S), Children's Healthcare of Atlanta (M.S.S), Woodruff Health Sciences Center 2020 COVID‐19 CURE Award (M.S.S), by the National Institutes of Health (NIH) through the National Institute for Allergy and Infectious Diseases under award numbers ORIP/OD P51OD011132 (M.S.S), 3U19AI057266‐17S1 (M.S.S.), HIPC COVID‐19 Supplement U19AI090023 (M.S.S), R01AI127799 (M.S.S.), R01AI148378 (M.S.S.); and by the Infectious Diseases Clinical Research Consortium UM1AI148684 (E.A., N.R., J.W., M.S.S.), R00AG049092 (V.D.M), and the World Reference Center for Emerging Viruses and Arboviruses R24AI120942 (V.D.M). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. 3 Publisher Copyright: {\textcopyright} 2020 Wiley Periodicals LLC",
year = "2020",
month = dec,
doi = "10.1002/cpim.116",
language = "English (US)",
volume = "131",
journal = "Current Protocols in Immunology",
issn = "1934-3671",
publisher = "John Wiley and Sons Inc.",
number = "1",
}