Development of a reverse transcription-polymerase chain reaction assay for diagnosis of lymphocytic choriomeningitis virus infection and its use in a prospective surveillance study

Jong Y. Park, C. J. Peters, Pierre E. Rollin, Thomas Ksiazek, Barry Gray, Ken B. Waites, Charles B. Stephensen

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. In this study, single-stage and nested reverse transcription-polymerase chain reaction (RT-PCR) assays were developed that targeted the GPC and N genes of LCMV. Both assays detected <1 TCID50 unit of LCMV. These assays were used to measure the incidence of LCMV infection by testing cerebrospinal fluid (CSF) samples with ≤10 leukocytes/μl collected over 1 year from patients undergoing lumbar puncture for diagnostic reasons at two Birmingham hospitals. Samples were tested for the presence of LCMV RNA by using the RT-PCR assay and for LCMV-specific IgM antibody by using an ELISA assay. None of the specimens collected from 813 patients was positive by either assay. Although no cases of acute infection were detected, 4% (11/272) of serum collected from a subset of patients was positive for LCMV-specific IgG. A significantly greater rate of seropositivity was found among subjects over 60 years of age (9.4%; P < 0.025) than was found in younger subjects (2.4% at 30-59 years of age; 0% at <30 years of age). These data suggest that serious central nervous system disease due to LCMV infection is not common in this population. The high rate of seropositivity in those over 60 years of age suggest that infection was once more common.

Original languageEnglish (US)
Pages (from-to)107-114
Number of pages8
JournalJournal of Medical Virology
Volume51
Issue number2
DOIs
StatePublished - Feb 1997
Externally publishedYes

Fingerprint

Lymphocytic choriomeningitis virus
Virus Diseases
Reverse Transcription
Prospective Studies
Polymerase Chain Reaction
Arenavirus
Spinal Puncture
Central Nervous System Diseases
Encephalitis
Infection
Meningitis
Immunoglobulin M
Cerebrospinal Fluid
Leukocytes
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
RNA
Antibodies
Incidence

Keywords

  • arenavirus
  • encephalitis
  • meningitis
  • serum antibody

ASJC Scopus subject areas

  • Virology

Cite this

Development of a reverse transcription-polymerase chain reaction assay for diagnosis of lymphocytic choriomeningitis virus infection and its use in a prospective surveillance study. / Park, Jong Y.; Peters, C. J.; Rollin, Pierre E.; Ksiazek, Thomas; Gray, Barry; Waites, Ken B.; Stephensen, Charles B.

In: Journal of Medical Virology, Vol. 51, No. 2, 02.1997, p. 107-114.

Research output: Contribution to journalArticle

Park, Jong Y. ; Peters, C. J. ; Rollin, Pierre E. ; Ksiazek, Thomas ; Gray, Barry ; Waites, Ken B. ; Stephensen, Charles B. / Development of a reverse transcription-polymerase chain reaction assay for diagnosis of lymphocytic choriomeningitis virus infection and its use in a prospective surveillance study. In: Journal of Medical Virology. 1997 ; Vol. 51, No. 2. pp. 107-114.
@article{0521e54bb17f445fb30b6b50c6af6b0d,
title = "Development of a reverse transcription-polymerase chain reaction assay for diagnosis of lymphocytic choriomeningitis virus infection and its use in a prospective surveillance study",
abstract = "Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. In this study, single-stage and nested reverse transcription-polymerase chain reaction (RT-PCR) assays were developed that targeted the GPC and N genes of LCMV. Both assays detected <1 TCID50 unit of LCMV. These assays were used to measure the incidence of LCMV infection by testing cerebrospinal fluid (CSF) samples with ≤10 leukocytes/μl collected over 1 year from patients undergoing lumbar puncture for diagnostic reasons at two Birmingham hospitals. Samples were tested for the presence of LCMV RNA by using the RT-PCR assay and for LCMV-specific IgM antibody by using an ELISA assay. None of the specimens collected from 813 patients was positive by either assay. Although no cases of acute infection were detected, 4{\%} (11/272) of serum collected from a subset of patients was positive for LCMV-specific IgG. A significantly greater rate of seropositivity was found among subjects over 60 years of age (9.4{\%}; P < 0.025) than was found in younger subjects (2.4{\%} at 30-59 years of age; 0{\%} at <30 years of age). These data suggest that serious central nervous system disease due to LCMV infection is not common in this population. The high rate of seropositivity in those over 60 years of age suggest that infection was once more common.",
keywords = "arenavirus, encephalitis, meningitis, serum antibody",
author = "Park, {Jong Y.} and Peters, {C. J.} and Rollin, {Pierre E.} and Thomas Ksiazek and Barry Gray and Waites, {Ken B.} and Stephensen, {Charles B.}",
year = "1997",
month = "2",
doi = "10.1002/(SICI)1096-9071(199702)51:2<107::AID-JMV4>3.0.CO;2-B",
language = "English (US)",
volume = "51",
pages = "107--114",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Development of a reverse transcription-polymerase chain reaction assay for diagnosis of lymphocytic choriomeningitis virus infection and its use in a prospective surveillance study

AU - Park, Jong Y.

AU - Peters, C. J.

AU - Rollin, Pierre E.

AU - Ksiazek, Thomas

AU - Gray, Barry

AU - Waites, Ken B.

AU - Stephensen, Charles B.

PY - 1997/2

Y1 - 1997/2

N2 - Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. In this study, single-stage and nested reverse transcription-polymerase chain reaction (RT-PCR) assays were developed that targeted the GPC and N genes of LCMV. Both assays detected <1 TCID50 unit of LCMV. These assays were used to measure the incidence of LCMV infection by testing cerebrospinal fluid (CSF) samples with ≤10 leukocytes/μl collected over 1 year from patients undergoing lumbar puncture for diagnostic reasons at two Birmingham hospitals. Samples were tested for the presence of LCMV RNA by using the RT-PCR assay and for LCMV-specific IgM antibody by using an ELISA assay. None of the specimens collected from 813 patients was positive by either assay. Although no cases of acute infection were detected, 4% (11/272) of serum collected from a subset of patients was positive for LCMV-specific IgG. A significantly greater rate of seropositivity was found among subjects over 60 years of age (9.4%; P < 0.025) than was found in younger subjects (2.4% at 30-59 years of age; 0% at <30 years of age). These data suggest that serious central nervous system disease due to LCMV infection is not common in this population. The high rate of seropositivity in those over 60 years of age suggest that infection was once more common.

AB - Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. In this study, single-stage and nested reverse transcription-polymerase chain reaction (RT-PCR) assays were developed that targeted the GPC and N genes of LCMV. Both assays detected <1 TCID50 unit of LCMV. These assays were used to measure the incidence of LCMV infection by testing cerebrospinal fluid (CSF) samples with ≤10 leukocytes/μl collected over 1 year from patients undergoing lumbar puncture for diagnostic reasons at two Birmingham hospitals. Samples were tested for the presence of LCMV RNA by using the RT-PCR assay and for LCMV-specific IgM antibody by using an ELISA assay. None of the specimens collected from 813 patients was positive by either assay. Although no cases of acute infection were detected, 4% (11/272) of serum collected from a subset of patients was positive for LCMV-specific IgG. A significantly greater rate of seropositivity was found among subjects over 60 years of age (9.4%; P < 0.025) than was found in younger subjects (2.4% at 30-59 years of age; 0% at <30 years of age). These data suggest that serious central nervous system disease due to LCMV infection is not common in this population. The high rate of seropositivity in those over 60 years of age suggest that infection was once more common.

KW - arenavirus

KW - encephalitis

KW - meningitis

KW - serum antibody

UR - http://www.scopus.com/inward/record.url?scp=0031053922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031053922&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9071(199702)51:2<107::AID-JMV4>3.0.CO;2-B

DO - 10.1002/(SICI)1096-9071(199702)51:2<107::AID-JMV4>3.0.CO;2-B

M3 - Article

VL - 51

SP - 107

EP - 114

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 2

ER -