Development of a transplantable model of pancreatic duct adenocarcinoma

Courtney M. Townsend, Raymond B. Franklin, Frank B. Gelder, Elena Glass, James C. Thompson

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

We report here the first development of a continuous cell line in tissue culture of an animal pancreatic duct adenocarcinoma that is histologically similar to human pancreatic duct adenocarcinoma. A primary pancreatic duct adenocarcinoma, induced in a male Syrian golden hamster after 23 weeks of weekly subcutaneous injections of N-nitrobis(2-hydroxypropyl)amine, was minced and injected subcutaneously into three hamsters. After 8 weeks, a single tumor was apparent. Subsequent passages of fragments into the cheek pouches were performed at 3- to 4-week intervals. After five passages, minced fragments of a tumor were placed in tissue culture. Colonies appeared by 7 days; an epitheloid cell line, without fibroblasts, was established by 60 days. Single-cell suspensions, injected into hamster cheek pouches or subcutaneously, produced tumors in a dose-dependent fashion. Spent culture medium of tissue culture cells and saline extracts of freshly excised tumors contained pancreatic oncofetal antigen-like activity.

Original languageEnglish (US)
Pages (from-to)72-78
Number of pages7
JournalSurgery
Volume92
Issue number1
StatePublished - Jul 1982

ASJC Scopus subject areas

  • Surgery

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    Townsend, C. M., Franklin, R. B., Gelder, F. B., Glass, E., & Thompson, J. C. (1982). Development of a transplantable model of pancreatic duct adenocarcinoma. Surgery, 92(1), 72-78.