Development of a transplantable model of pancreatic duct adenocarcinoma

Courtney Townsend, Raymond B. Franklin, Frank B. Gelder, Elena Glass, James C. Thompson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We report here the first development of a continuous cell line in tissue culture of an animal pancreatic duct adenocarcinoma that is histologically similar to human pancreatic duct adenocarcinoma. A primary pancreatic duct adenocarcinoma, induced in a male Syrian golden hamster after 23 weeks of weekly subcutaneous injections of N-nitrobis(2-hydroxypropyl)amine, was minced and injected subcutaneously into three hamsters. After 8 weeks, a single tumor was apparent. Subsequent passages of fragments into the cheek pouches were performed at 3- to 4-week intervals. After five passages, minced fragments of a tumor were placed in tissue culture. Colonies appeared by 7 days; an epitheloid cell line, without fibroblasts, was established by 60 days. Single-cell suspensions, injected into hamster cheek pouches or subcutaneously, produced tumors in a dose-dependent fashion. Spent culture medium of tissue culture cells and saline extracts of freshly excised tumors contained pancreatic oncofetal antigen-like activity.

Original languageEnglish (US)
Pages (from-to)72-78
Number of pages7
JournalSurgery
Volume92
Issue number1
StatePublished - 1982

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Pancreatic Ducts
Adenocarcinoma
Cheek
Cricetinae
Neoplasms
Cell Line
Mesocricetus
Subcutaneous Injections
Cell Extracts
Amines
Culture Media
Suspensions
Fibroblasts

ASJC Scopus subject areas

  • Surgery

Cite this

Townsend, C., Franklin, R. B., Gelder, F. B., Glass, E., & Thompson, J. C. (1982). Development of a transplantable model of pancreatic duct adenocarcinoma. Surgery, 92(1), 72-78.

Development of a transplantable model of pancreatic duct adenocarcinoma. / Townsend, Courtney; Franklin, Raymond B.; Gelder, Frank B.; Glass, Elena; Thompson, James C.

In: Surgery, Vol. 92, No. 1, 1982, p. 72-78.

Research output: Contribution to journalArticle

Townsend, C, Franklin, RB, Gelder, FB, Glass, E & Thompson, JC 1982, 'Development of a transplantable model of pancreatic duct adenocarcinoma', Surgery, vol. 92, no. 1, pp. 72-78.
Townsend C, Franklin RB, Gelder FB, Glass E, Thompson JC. Development of a transplantable model of pancreatic duct adenocarcinoma. Surgery. 1982;92(1):72-78.
Townsend, Courtney ; Franklin, Raymond B. ; Gelder, Frank B. ; Glass, Elena ; Thompson, James C. / Development of a transplantable model of pancreatic duct adenocarcinoma. In: Surgery. 1982 ; Vol. 92, No. 1. pp. 72-78.
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