Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells

Tetsuro Ikegami; Masayuki Saijo; Masahiro Niikura; Mary E.G. Miranda; Alan B. Calaor; Marvin Hernandez; Daria L. Manalo; Ichiro Kurane; Yasuhiro Yoshikawa; Shigeru Morikawa

doi:10.1111/j.1348-0421.2002.tb02745.x

Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells

Tetsuro Ikegami, Masayuki Saijo, Masahiro Niikura, Mary E.G. Miranda, Alan B. Calaor, Marvin Hernandez, Daria L. Manalo, Ichiro Kurane, Yasuhiro Yoshikawa, Shigeru Morikawa

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24 Scopus citations

Abstract

An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.

Original language	English (US)
Pages (from-to)	633-638
Number of pages	6
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	46
Issue number	9
DOIs	https://doi.org/10.1111/j.1348-0421.2002.tb02745.x
State	Published - 2002
Externally published	Yes

Keywords

Cynomolgus macaque
Ebola virus subtype Reston
IFA
Recombinant nucleoprotein

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.2002.tb02745.x

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Ikegami, T., Saijo, M., Niikura, M., Miranda, M. E. G., Calaor, A. B., Hernandez, M., Manalo, D. L., Kurane, I., Yoshikawa, Y., & Morikawa, S. (2002). Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells. MICROBIOLOGY and IMMUNOLOGY, 46(9), 633-638. https://doi.org/10.1111/j.1348-0421.2002.tb02745.x

Ikegami, T, Saijo, M, Niikura, M, Miranda, MEG, Calaor, AB, Hernandez, M, Manalo, DL, Kurane, I, Yoshikawa, Y & Morikawa, S 2002, 'Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells', MICROBIOLOGY and IMMUNOLOGY, vol. 46, no. 9, pp. 633-638. https://doi.org/10.1111/j.1348-0421.2002.tb02745.x

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abstract = "An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.",

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author = "Tetsuro Ikegami and Masayuki Saijo and Masahiro Niikura and Miranda, {Mary E.G.} and Calaor, {Alan B.} and Marvin Hernandez and Manalo, {Daria L.} and Ichiro Kurane and Yasuhiro Yoshikawa and Shigeru Morikawa",

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doi = "10.1111/j.1348-0421.2002.tb02745.x",

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AU - Ikegami, Tetsuro

AU - Saijo, Masayuki

AU - Niikura, Masahiro

AU - Miranda, Mary E.G.

AU - Calaor, Alan B.

AU - Hernandez, Marvin

AU - Manalo, Daria L.

AU - Kurane, Ichiro

AU - Yoshikawa, Yasuhiro

AU - Morikawa, Shigeru

PY - 2002

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N2 - An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.

AB - An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.

KW - Cynomolgus macaque

KW - Ebola virus subtype Reston

KW - IFA

KW - Recombinant nucleoprotein

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Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells

Abstract

Keywords

ASJC Scopus subject areas

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