Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells

Tetsuro Ikegami, Masayuki Saijo, Masahiro Niikura, Mary E.G. Miranda, Alan B. Calaor, Marvin Hernandez, Daria L. Manalo, Ichiro Kurane, Yasuhiro Yoshikawa, Shigeru Morikawa

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18 Scopus citations


An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.

Original languageEnglish (US)
Pages (from-to)633-638
Number of pages6
Issue number9
StatePublished - Jan 1 2002
Externally publishedYes



  • Cynomolgus macaque
  • Ebola virus subtype Reston
  • IFA
  • Recombinant nucleoprotein

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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