Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells

Tetsuro Ikegami, Masayuki Saijo, Masahiro Niikura, Mary E G Miranda, Alan B. Calaor, Marvin Hernandez, Daria L. Manalo, Ichiro Kurane, Yasuhiro Yoshikawa, Shigeru Morikawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.

Original languageEnglish (US)
Pages (from-to)633-638
Number of pages6
JournalMicrobiology and Immunology
Volume46
Issue number9
StatePublished - 2002
Externally publishedYes

Fingerprint

Ebolavirus
Nucleoproteins
HeLa Cells
Haplorhini
Fluorescent Antibody Technique
Philippines
Antibodies
Seroepidemiologic Studies
Serum
Disease Outbreaks
Immunoglobulin G
Rabbits
Cell Line
Infection

Keywords

  • Cynomolgus macaque
  • Ebola virus subtype Reston
  • IFA
  • Recombinant nucleoprotein

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells. / Ikegami, Tetsuro; Saijo, Masayuki; Niikura, Masahiro; Miranda, Mary E G; Calaor, Alan B.; Hernandez, Marvin; Manalo, Daria L.; Kurane, Ichiro; Yoshikawa, Yasuhiro; Morikawa, Shigeru.

In: Microbiology and Immunology, Vol. 46, No. 9, 2002, p. 633-638.

Research output: Contribution to journalArticle

Ikegami, T, Saijo, M, Niikura, M, Miranda, MEG, Calaor, AB, Hernandez, M, Manalo, DL, Kurane, I, Yoshikawa, Y & Morikawa, S 2002, 'Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells', Microbiology and Immunology, vol. 46, no. 9, pp. 633-638.
Ikegami, Tetsuro ; Saijo, Masayuki ; Niikura, Masahiro ; Miranda, Mary E G ; Calaor, Alan B. ; Hernandez, Marvin ; Manalo, Daria L. ; Kurane, Ichiro ; Yoshikawa, Yasuhiro ; Morikawa, Shigeru. / Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells. In: Microbiology and Immunology. 2002 ; Vol. 46, No. 9. pp. 633-638.
@article{7fdcc3de27a4455389c57e7c1377ac17,
title = "Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells",
abstract = "An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.",
keywords = "Cynomolgus macaque, Ebola virus subtype Reston, IFA, Recombinant nucleoprotein",
author = "Tetsuro Ikegami and Masayuki Saijo and Masahiro Niikura and Miranda, {Mary E G} and Calaor, {Alan B.} and Marvin Hernandez and Manalo, {Daria L.} and Ichiro Kurane and Yasuhiro Yoshikawa and Shigeru Morikawa",
year = "2002",
language = "English (US)",
volume = "46",
pages = "633--638",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "9",

}

TY - JOUR

T1 - Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells

AU - Ikegami, Tetsuro

AU - Saijo, Masayuki

AU - Niikura, Masahiro

AU - Miranda, Mary E G

AU - Calaor, Alan B.

AU - Hernandez, Marvin

AU - Manalo, Daria L.

AU - Kurane, Ichiro

AU - Yoshikawa, Yasuhiro

AU - Morikawa, Shigeru

PY - 2002

Y1 - 2002

N2 - An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.

AB - An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) anti-bodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.

KW - Cynomolgus macaque

KW - Ebola virus subtype Reston

KW - IFA

KW - Recombinant nucleoprotein

UR - http://www.scopus.com/inward/record.url?scp=0036379078&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036379078&partnerID=8YFLogxK

M3 - Article

C2 - 12437031

AN - SCOPUS:0036379078

VL - 46

SP - 633

EP - 638

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 9

ER -