Development of cell lines after exposure of chicken embryonic fibroblasts to Herpes simplex virus type 2 at supraoptimal temperature

L. Geder, L. Vaczi, Istvan Boldogh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chicken embryonic fibroblasts were exposed to Herpes simplex virus type 1 (HSV 1) and type 2 (HSV 2). Incubation at 40° C for prolonged periods inhibited the production of infectious HSV 2 without preventing most of the cells from degeneration. This procedure, however, resulted in rapid growth of a few cells which survived infection and showed no cytopathic changes and synthesis of infectious virus. Two isolated clones grew into continuous cell lines. Both showed increased resistance to HSV 2 infection but proved susceptible to HSV 1. HSV specific antigens were found in 5-10% of the cells by the indirect immunofluorescent technique. These chicken cell lines presented an opportunity to study the effect of HSV 2 cells under non permissive conditions.

Original languageEnglish (US)
Pages (from-to)119-125
Number of pages7
JournalActa Microbiologica Academiae Scientiarum Hungaricae
Volume20
Issue number2
StatePublished - 1973
Externally publishedYes

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Human Herpesvirus 2
Human Herpesvirus 1
Chickens
Fibroblasts
Cell Line
Temperature
Infection
Clone Cells
Viruses
Antigens
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "Chicken embryonic fibroblasts were exposed to Herpes simplex virus type 1 (HSV 1) and type 2 (HSV 2). Incubation at 40° C for prolonged periods inhibited the production of infectious HSV 2 without preventing most of the cells from degeneration. This procedure, however, resulted in rapid growth of a few cells which survived infection and showed no cytopathic changes and synthesis of infectious virus. Two isolated clones grew into continuous cell lines. Both showed increased resistance to HSV 2 infection but proved susceptible to HSV 1. HSV specific antigens were found in 5-10{\%} of the cells by the indirect immunofluorescent technique. These chicken cell lines presented an opportunity to study the effect of HSV 2 cells under non permissive conditions.",
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AU - Geder, L.

AU - Vaczi, L.

AU - Boldogh, Istvan

PY - 1973

Y1 - 1973

N2 - Chicken embryonic fibroblasts were exposed to Herpes simplex virus type 1 (HSV 1) and type 2 (HSV 2). Incubation at 40° C for prolonged periods inhibited the production of infectious HSV 2 without preventing most of the cells from degeneration. This procedure, however, resulted in rapid growth of a few cells which survived infection and showed no cytopathic changes and synthesis of infectious virus. Two isolated clones grew into continuous cell lines. Both showed increased resistance to HSV 2 infection but proved susceptible to HSV 1. HSV specific antigens were found in 5-10% of the cells by the indirect immunofluorescent technique. These chicken cell lines presented an opportunity to study the effect of HSV 2 cells under non permissive conditions.

AB - Chicken embryonic fibroblasts were exposed to Herpes simplex virus type 1 (HSV 1) and type 2 (HSV 2). Incubation at 40° C for prolonged periods inhibited the production of infectious HSV 2 without preventing most of the cells from degeneration. This procedure, however, resulted in rapid growth of a few cells which survived infection and showed no cytopathic changes and synthesis of infectious virus. Two isolated clones grew into continuous cell lines. Both showed increased resistance to HSV 2 infection but proved susceptible to HSV 1. HSV specific antigens were found in 5-10% of the cells by the indirect immunofluorescent technique. These chicken cell lines presented an opportunity to study the effect of HSV 2 cells under non permissive conditions.

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JO - Acta Microbiologica et Immunologica Hungarica

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