Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2

Nigel Bourne; Brianne N. Banasik; Clarice L. Perry; Aaron L. Miller; Mellodee White; Richard B. Pyles; Gregg N. Milligan

doi:10.1016/j.virol.2018.10.023

Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2

Nigel Bourne, Brianne N. Banasik, Clarice L. Perry, Aaron L. Miller, Mellodee White, Richard B. Pyles, Gregg N. Milligan

Pediatrics

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Most analyses of genital immunity to herpes simplex virus type 2 (HSV-2) have been performed in females, consequently immune protection of the male genital epithelium is incompletely understood. We developed a model of male genital HSV-2 infection resulting from intrarectal inoculation of guinea pigs. Vesicular lesions developed transiently on the perineum and foreskin concurrent with acute virus shedding. Virus shedding and recurrent genital lesions were also detected after establishment of a latent infection. Analysis of perineum and foreskin RNA detected transcripts for IFNγ, proinflammatory and regulatory cytokines, and for genes involved in migration and regulation of leukocytes. HSV-specific T cells were detected in lymphoid and genital tissues after resolution of the primary infection whereas virus-specific antibody secreting cells were detected only in lymphoid tissue.

Original language	English (US)
Pages (from-to)	180-188
Number of pages	9
Journal	Virology
Volume	526
DOIs	https://doi.org/10.1016/j.virol.2018.10.023
State	Published - Jan 2 2019

Keywords

Foreskin
Gene expression
HSV-2
IFNγ
Male guinea pig
Microarray
Perineum

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2018.10.023

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title = "Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2",

abstract = "Most analyses of genital immunity to herpes simplex virus type 2 (HSV-2) have been performed in females, consequently immune protection of the male genital epithelium is incompletely understood. We developed a model of male genital HSV-2 infection resulting from intrarectal inoculation of guinea pigs. Vesicular lesions developed transiently on the perineum and foreskin concurrent with acute virus shedding. Virus shedding and recurrent genital lesions were also detected after establishment of a latent infection. Analysis of perineum and foreskin RNA detected transcripts for IFNγ, proinflammatory and regulatory cytokines, and for genes involved in migration and regulation of leukocytes. HSV-specific T cells were detected in lymphoid and genital tissues after resolution of the primary infection whereas virus-specific antibody secreting cells were detected only in lymphoid tissue.",

keywords = "Foreskin, Gene expression, HSV-2, IFNγ, Male guinea pig, Microarray, Perineum",

author = "Nigel Bourne and Banasik, {Brianne N.} and Perry, {Clarice L.} and Miller, {Aaron L.} and Mellodee White and Pyles, {Richard B.} and Milligan, {Gregg N.}",

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doi = "10.1016/j.virol.2018.10.023",

language = "English (US)",

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T1 - Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2

AU - Bourne, Nigel

AU - Banasik, Brianne N.

AU - Perry, Clarice L.

AU - Miller, Aaron L.

AU - White, Mellodee

AU - Pyles, Richard B.

AU - Milligan, Gregg N.

PY - 2019/1/2

Y1 - 2019/1/2

N2 - Most analyses of genital immunity to herpes simplex virus type 2 (HSV-2) have been performed in females, consequently immune protection of the male genital epithelium is incompletely understood. We developed a model of male genital HSV-2 infection resulting from intrarectal inoculation of guinea pigs. Vesicular lesions developed transiently on the perineum and foreskin concurrent with acute virus shedding. Virus shedding and recurrent genital lesions were also detected after establishment of a latent infection. Analysis of perineum and foreskin RNA detected transcripts for IFNγ, proinflammatory and regulatory cytokines, and for genes involved in migration and regulation of leukocytes. HSV-specific T cells were detected in lymphoid and genital tissues after resolution of the primary infection whereas virus-specific antibody secreting cells were detected only in lymphoid tissue.

AB - Most analyses of genital immunity to herpes simplex virus type 2 (HSV-2) have been performed in females, consequently immune protection of the male genital epithelium is incompletely understood. We developed a model of male genital HSV-2 infection resulting from intrarectal inoculation of guinea pigs. Vesicular lesions developed transiently on the perineum and foreskin concurrent with acute virus shedding. Virus shedding and recurrent genital lesions were also detected after establishment of a latent infection. Analysis of perineum and foreskin RNA detected transcripts for IFNγ, proinflammatory and regulatory cytokines, and for genes involved in migration and regulation of leukocytes. HSV-specific T cells were detected in lymphoid and genital tissues after resolution of the primary infection whereas virus-specific antibody secreting cells were detected only in lymphoid tissue.

KW - Foreskin

KW - Gene expression

KW - HSV-2

KW - IFNγ

KW - Male guinea pig

KW - Microarray

KW - Perineum

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JO - Virology

JF - Virology

ER -

Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2

Abstract

Keywords

ASJC Scopus subject areas

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