Development of Endotrypanum (Kinetoplastida

Trypanosomatidae) in Experimentally Infected Phlebotomine Sand Flies (Diptera: Psychodidae)

Antonia Maria Ramos Franco, Robert B. Tesh, Hilda Guzman, Maria P. Deane, Gabriel Grimaldi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The developmental biology (parasite establishment, migration, and differentiation) of Brazilian strains of Endotrypanum are reported for 3 sand fly species: Lutzomyia longipalpis Lutz & Neiva, L. shannoni Dyar, and Phlebotomus papatasi Scopoli. Laboratory-reared sand flies were infected by feeding on a promastigote suspension through a chick-skin membrane. Infections within the insect gut were examined at various times after feeding by staining fresh and fixed specimens. Development of Endotrypanum varied for each parasite-host species association. After feeding on culture forms of E. schaudinni Mesnil & Brimont (strain ISHA/BR/80/IM1111), significantly more L. shannoni (100%, 9/9) became infected than did L. longipalpis (62.3%, 33/53) or P. papatasi (27.3%, 15/55). The greatest number of infections were in the midgut and hindgut from 6 to 16 d after feeding, but flagellates also were present in the Malpighian tubules. Moreover, distinct development patterns in the sand fly gut were obtained when the Callejon L. longipalpis colony was fed on cultures of other Endotrypanum strains. Significantly fewer sand flies became infected with strain MCHO/BR/85/ IM2259 (18.2%, 4/22) than with strain ISHA/BR/80/IM1111 (55.6%, 20/36). There were also individual variation in the distribution and survival of parasites within the guts of flies in each group. These data indicate that there is variation in the susceptibility to infection with Endotrypanum among and within sand fly species.

Original languageEnglish (US)
Pages (from-to)189-192
Number of pages4
JournalJournal of Medical Entomology
Volume34
Issue number2
StatePublished - Mar 1997

Fingerprint

Endotrypanum
Kinetoplastida
Trypanosomatina
Trypanosomatidae
Psychodidae
Phlebotominae
Diptera
Lutzomyia longipalpis
Phlebotomus papatasi
digestive system
Parasites
parasites
Lutzomyia shannoni
infection
promastigotes
Infection
Malpighian Tubules
Malpighian tubules
Phlebotomus
hindgut

Keywords

  • Endotrypanum
  • Experimental infection
  • Sand flies
  • Trypanosomatidae

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Development of Endotrypanum (Kinetoplastida : Trypanosomatidae) in Experimentally Infected Phlebotomine Sand Flies (Diptera: Psychodidae). / Franco, Antonia Maria Ramos; Tesh, Robert B.; Guzman, Hilda; Deane, Maria P.; Grimaldi, Gabriel.

In: Journal of Medical Entomology, Vol. 34, No. 2, 03.1997, p. 189-192.

Research output: Contribution to journalArticle

Franco, Antonia Maria Ramos ; Tesh, Robert B. ; Guzman, Hilda ; Deane, Maria P. ; Grimaldi, Gabriel. / Development of Endotrypanum (Kinetoplastida : Trypanosomatidae) in Experimentally Infected Phlebotomine Sand Flies (Diptera: Psychodidae). In: Journal of Medical Entomology. 1997 ; Vol. 34, No. 2. pp. 189-192.
@article{5ffd8c66bcf84752a9ff7414dbc1f7e9,
title = "Development of Endotrypanum (Kinetoplastida: Trypanosomatidae) in Experimentally Infected Phlebotomine Sand Flies (Diptera: Psychodidae)",
abstract = "The developmental biology (parasite establishment, migration, and differentiation) of Brazilian strains of Endotrypanum are reported for 3 sand fly species: Lutzomyia longipalpis Lutz & Neiva, L. shannoni Dyar, and Phlebotomus papatasi Scopoli. Laboratory-reared sand flies were infected by feeding on a promastigote suspension through a chick-skin membrane. Infections within the insect gut were examined at various times after feeding by staining fresh and fixed specimens. Development of Endotrypanum varied for each parasite-host species association. After feeding on culture forms of E. schaudinni Mesnil & Brimont (strain ISHA/BR/80/IM1111), significantly more L. shannoni (100{\%}, 9/9) became infected than did L. longipalpis (62.3{\%}, 33/53) or P. papatasi (27.3{\%}, 15/55). The greatest number of infections were in the midgut and hindgut from 6 to 16 d after feeding, but flagellates also were present in the Malpighian tubules. Moreover, distinct development patterns in the sand fly gut were obtained when the Callejon L. longipalpis colony was fed on cultures of other Endotrypanum strains. Significantly fewer sand flies became infected with strain MCHO/BR/85/ IM2259 (18.2{\%}, 4/22) than with strain ISHA/BR/80/IM1111 (55.6{\%}, 20/36). There were also individual variation in the distribution and survival of parasites within the guts of flies in each group. These data indicate that there is variation in the susceptibility to infection with Endotrypanum among and within sand fly species.",
keywords = "Endotrypanum, Experimental infection, Sand flies, Trypanosomatidae",
author = "Franco, {Antonia Maria Ramos} and Tesh, {Robert B.} and Hilda Guzman and Deane, {Maria P.} and Gabriel Grimaldi",
year = "1997",
month = "3",
language = "English (US)",
volume = "34",
pages = "189--192",
journal = "Journal of Medical Entomology",
issn = "0022-2585",
publisher = "Entomological Society of America",
number = "2",

}

TY - JOUR

T1 - Development of Endotrypanum (Kinetoplastida

T2 - Trypanosomatidae) in Experimentally Infected Phlebotomine Sand Flies (Diptera: Psychodidae)

AU - Franco, Antonia Maria Ramos

AU - Tesh, Robert B.

AU - Guzman, Hilda

AU - Deane, Maria P.

AU - Grimaldi, Gabriel

PY - 1997/3

Y1 - 1997/3

N2 - The developmental biology (parasite establishment, migration, and differentiation) of Brazilian strains of Endotrypanum are reported for 3 sand fly species: Lutzomyia longipalpis Lutz & Neiva, L. shannoni Dyar, and Phlebotomus papatasi Scopoli. Laboratory-reared sand flies were infected by feeding on a promastigote suspension through a chick-skin membrane. Infections within the insect gut were examined at various times after feeding by staining fresh and fixed specimens. Development of Endotrypanum varied for each parasite-host species association. After feeding on culture forms of E. schaudinni Mesnil & Brimont (strain ISHA/BR/80/IM1111), significantly more L. shannoni (100%, 9/9) became infected than did L. longipalpis (62.3%, 33/53) or P. papatasi (27.3%, 15/55). The greatest number of infections were in the midgut and hindgut from 6 to 16 d after feeding, but flagellates also were present in the Malpighian tubules. Moreover, distinct development patterns in the sand fly gut were obtained when the Callejon L. longipalpis colony was fed on cultures of other Endotrypanum strains. Significantly fewer sand flies became infected with strain MCHO/BR/85/ IM2259 (18.2%, 4/22) than with strain ISHA/BR/80/IM1111 (55.6%, 20/36). There were also individual variation in the distribution and survival of parasites within the guts of flies in each group. These data indicate that there is variation in the susceptibility to infection with Endotrypanum among and within sand fly species.

AB - The developmental biology (parasite establishment, migration, and differentiation) of Brazilian strains of Endotrypanum are reported for 3 sand fly species: Lutzomyia longipalpis Lutz & Neiva, L. shannoni Dyar, and Phlebotomus papatasi Scopoli. Laboratory-reared sand flies were infected by feeding on a promastigote suspension through a chick-skin membrane. Infections within the insect gut were examined at various times after feeding by staining fresh and fixed specimens. Development of Endotrypanum varied for each parasite-host species association. After feeding on culture forms of E. schaudinni Mesnil & Brimont (strain ISHA/BR/80/IM1111), significantly more L. shannoni (100%, 9/9) became infected than did L. longipalpis (62.3%, 33/53) or P. papatasi (27.3%, 15/55). The greatest number of infections were in the midgut and hindgut from 6 to 16 d after feeding, but flagellates also were present in the Malpighian tubules. Moreover, distinct development patterns in the sand fly gut were obtained when the Callejon L. longipalpis colony was fed on cultures of other Endotrypanum strains. Significantly fewer sand flies became infected with strain MCHO/BR/85/ IM2259 (18.2%, 4/22) than with strain ISHA/BR/80/IM1111 (55.6%, 20/36). There were also individual variation in the distribution and survival of parasites within the guts of flies in each group. These data indicate that there is variation in the susceptibility to infection with Endotrypanum among and within sand fly species.

KW - Endotrypanum

KW - Experimental infection

KW - Sand flies

KW - Trypanosomatidae

UR - http://www.scopus.com/inward/record.url?scp=0031090788&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031090788&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 189

EP - 192

JO - Journal of Medical Entomology

JF - Journal of Medical Entomology

SN - 0022-2585

IS - 2

ER -