TY - JOUR
T1 - Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals
AU - Ouarti, Basma
AU - Laroche, Maureen
AU - Righi, Souad
AU - Meguini, Mohamed Nadir
AU - Benakhla, Ahmed
AU - Raoult, Didier
AU - Parola, Philippe
N1 - Funding Information:
Acknowledgements. We are grateful to all the people who supported this study. We also thank Azzedine Ouarti who kindly helped us to access livestock, local guides and lice collection to facilitate our work. This study was supported by the Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, the French National Research Agency under the “Investissements d’avenir” program, reference ANR-10-IAHU-03, the Provence-Alpes-Côte-d’Azur region, and European ERDF PRIMI funding.
Publisher Copyright:
© 2020 B. Ouarti et al., published by EDP Sciences.
PY - 2020
Y1 - 2020
N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis and laboratory reared Pediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.
AB - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis and laboratory reared Pediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.
KW - Anoplura
KW - Lice
KW - MALDI-TOF MS
KW - Mallophaga
KW - Phthiraptera
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U2 - 10.1051/parasite/2020026
DO - 10.1051/parasite/2020026
M3 - Article
C2 - 32351208
AN - SCOPUS:85084170510
SN - 1252-607X
VL - 27
JO - Parasite
JF - Parasite
M1 - 27
ER -