Development of purinergic sensitivity in sensory neurons after peripheral nerve injury in the rat

Junli Zhou, Kyungsoon Chung, Jin Chung

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Purinoceptors are present in the cell bodies as well as in both peripheral and central terminals of many sensory neurons, where they may play a role in sensory transmission, including pain. After peripheral nerve injury at the spinal nerve level, some axotomized afferent neurons develop ongoing discharges (ectopic discharges) that originate in the dorsal root ganglion (DRG). In the present study, we attempted to determine whether or not purinergic sensitivity develops in injured sensory neurons which display ectopic discharges, as well as in silent units. The L4 and L5 spinal nerves were ligated in Sprague-Dawley rats. Four to 21 days after the surgery, the DRGs with attached dorsal roots and spinal nerves were removed and ectopic discharges were recorded from teased dorsal root fascicles using an in vitro recording set-up. The results showed that 75.6 and 65.1% of the chronically axotomized DRG neurons displaying ectopic discharges enhanced their activity after application of adenosine 5′-triphosphate (ATP, 1 mM) or α,β-methylene ATP (mATP, 100 μM), respectively. In addition, application of these purinoceptor agonists evoked activity in 7 of 28 axotomized DRG neurons, which did not show ongoing discharges. In contrast, only 1 of 34 DRG neurons acutely isolated from normal rats (no previous spinal nerve ligation) responded to either mATP or ATP. In most of the tested units, mATP-induced enhancement of ectopic discharges was blocked by non-specific P2X receptor antagonists, PPADS or suramin. The data from the present study suggest that purinergic sensitivity develops in DRG neurons after chronic axotomy and that this purinergic sensitivity is likely to be mediated by P2X purinoceptors. This acquired purinergic sensitivity may play an important functional role in the enhancement of ectopic discharges and exacerbation of pain upon sympathetic activation in the neuropathic pain state.

Original languageEnglish (US)
Pages (from-to)161-169
Number of pages9
JournalBrain Research
Volume915
Issue number2
DOIs
StatePublished - Oct 12 2001

Fingerprint

Peripheral Nerve Injuries
Spinal Ganglia
Sensory Receptor Cells
Spinal Nerves
Adenosine Triphosphate
Neurons
Spinal Nerve Roots
Purinergic P2X Receptors
Purinergic Agonists
Purinergic Receptors
Suramin
Axotomy
Afferent Neurons
Pain
Diagnosis-Related Groups
Neuralgia
Ambulatory Surgical Procedures
Ligation
Sprague Dawley Rats

Keywords

  • α,β-Methylene ATP
  • Dorsal root ganglion
  • Ectopic discharge
  • Neuropathic pain
  • P2X purinoceptor
  • PPADS
  • Spinal nerve injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Development of purinergic sensitivity in sensory neurons after peripheral nerve injury in the rat. / Zhou, Junli; Chung, Kyungsoon; Chung, Jin.

In: Brain Research, Vol. 915, No. 2, 12.10.2001, p. 161-169.

Research output: Contribution to journalArticle

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