Development of reagents and assays for the detection of pathogenic Burkholderia species

Omar Qazi, Mridula Rani, Annie J. Gnanam, Thomas W. Cullen, Christopher M. Stead, Haley Kensing, Kate McCaul, Sarah Ngugi, Joann L. Prior, Alexandria Lipka, Judit M. Nagy, Gregory C. Whitlock, Barbara M. Judy, Sarah V. Harding, Richard W. Titball, Sachdev S. Sidhu, M. Stephen Trent, G. Barrie Kitto, Alfredo Torres, D. Mark EstesBrent Iverson, George Georgiou, Katherine A. Brown

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderia macromolecules and describe the generation of a Fab molecule targeted to the B. mallei BimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.

Original languageEnglish (US)
Pages (from-to)23-36
Number of pages14
JournalFaraday Discussions
Volume149
DOIs
StatePublished - Jan 17 2011

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

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    Qazi, O., Rani, M., Gnanam, A. J., Cullen, T. W., Stead, C. M., Kensing, H., McCaul, K., Ngugi, S., Prior, J. L., Lipka, A., Nagy, J. M., Whitlock, G. C., Judy, B. M., Harding, S. V., Titball, R. W., Sidhu, S. S., Trent, M. S., Kitto, G. B., Torres, A., ... Brown, K. A. (2011). Development of reagents and assays for the detection of pathogenic Burkholderia species. Faraday Discussions, 149, 23-36. https://doi.org/10.1039/c005422b