Development of reagents and assays for the detection of pathogenic Burkholderia species

Omar Qazi; Mridula Rani; Annie J. Gnanam; Thomas W. Cullen; Christopher M. Stead; Haley Kensing; Kate McCaul; Sarah Ngugi; Joann L. Prior; Alexandria Lipka; Judit M. Nagy; Gregory C. Whitlock; Barbara M. Judy; Sarah V. Harding; Richard W. Titball; Sachdev S. Sidhu; M. Stephen Trent; G. Barrie Kitto; Alfredo Torres; D. Mark Estes; Brent Iverson; George Georgiou; Katherine A. Brown

doi:10.1039/c005422b

Development of reagents and assays for the detection of pathogenic Burkholderia species

Omar Qazi, Mridula Rani, Annie J. Gnanam, Thomas W. Cullen, Christopher M. Stead, Haley Kensing, Kate McCaul, Sarah Ngugi, Joann L. Prior, Alexandria Lipka, Judit M. Nagy, Gregory C. Whitlock, Barbara M. Judy, Sarah V. Harding, Richard W. Titball, Sachdev S. Sidhu, M. Stephen Trent, G. Barrie Kitto, Alfredo Torres, D. Mark EstesBrent Iverson, George Georgiou, Katherine A. Brown

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderia macromolecules and describe the generation of a Fab molecule targeted to the B. mallei BimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.

Original language	English (US)
Pages (from-to)	23-36
Number of pages	14
Journal	Faraday Discussions
Volume	149
DOIs	https://doi.org/10.1039/c005422b
State	Published - 2011
Externally published	Yes

ASJC Scopus subject areas

Physical and Theoretical Chemistry

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10.1039/c005422b

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Qazi, O., Rani, M., Gnanam, A. J., Cullen, T. W., Stead, C. M., Kensing, H., McCaul, K., Ngugi, S., Prior, J. L., Lipka, A., Nagy, J. M., Whitlock, G. C., Judy, B. M., Harding, S. V., Titball, R. W., Sidhu, S. S., Trent, M. S., Kitto, G. B., Torres, A., ... Brown, K. A. (2011). Development of reagents and assays for the detection of pathogenic Burkholderia species. Faraday Discussions, 149, 23-36. https://doi.org/10.1039/c005422b

Qazi, O, Rani, M, Gnanam, AJ, Cullen, TW, Stead, CM, Kensing, H, McCaul, K, Ngugi, S, Prior, JL, Lipka, A, Nagy, JM, Whitlock, GC, Judy, BM, Harding, SV, Titball, RW, Sidhu, SS, Trent, MS, Kitto, GB, Torres, A, Estes, DM, Iverson, B, Georgiou, G & Brown, KA 2011, 'Development of reagents and assays for the detection of pathogenic Burkholderia species', Faraday Discussions, vol. 149, pp. 23-36. https://doi.org/10.1039/c005422b

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title = "Development of reagents and assays for the detection of pathogenic Burkholderia species",

abstract = "Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderia macromolecules and describe the generation of a Fab molecule targeted to the B. mallei BimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.",

author = "Omar Qazi and Mridula Rani and Gnanam, {Annie J.} and Cullen, {Thomas W.} and Stead, {Christopher M.} and Haley Kensing and Kate McCaul and Sarah Ngugi and Prior, {Joann L.} and Alexandria Lipka and Nagy, {Judit M.} and Whitlock, {Gregory C.} and Judy, {Barbara M.} and Harding, {Sarah V.} and Titball, {Richard W.} and Sidhu, {Sachdev S.} and Trent, {M. Stephen} and Kitto, {G. Barrie} and Alfredo Torres and Estes, {D. Mark} and Brent Iverson and George Georgiou and Brown, {Katherine A.}",

year = "2011",

doi = "10.1039/c005422b",

language = "English (US)",

volume = "149",

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AU - Qazi, Omar

AU - Rani, Mridula

AU - Gnanam, Annie J.

AU - Cullen, Thomas W.

AU - Stead, Christopher M.

AU - Kensing, Haley

AU - McCaul, Kate

AU - Ngugi, Sarah

AU - Prior, Joann L.

AU - Lipka, Alexandria

AU - Nagy, Judit M.

AU - Whitlock, Gregory C.

AU - Judy, Barbara M.

AU - Harding, Sarah V.

AU - Titball, Richard W.

AU - Sidhu, Sachdev S.

AU - Trent, M. Stephen

AU - Kitto, G. Barrie

AU - Torres, Alfredo

AU - Estes, D. Mark

AU - Iverson, Brent

AU - Georgiou, George

AU - Brown, Katherine A.

PY - 2011

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AB - Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderia macromolecules and describe the generation of a Fab molecule targeted to the B. mallei BimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.

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JO - Faraday Discussions

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Development of reagents and assays for the detection of pathogenic Burkholderia species

Abstract

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