Development of T-cell lines expressing functional HIV-1 envelope glycoproteins for evaluation of immune responses in HIV-infected individuals

Tibor Keler, Hongbo Li, Miles W. Cloyd, Laura A. Vitale, Yashwant M. Deo

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The human T-lymphoid cell line, CEM, was transfected with gp160 cDNA of human immunodeficiency virus type I (HIV-1)(pm213). Three clones expressing the envelope glycoproteins (env), designated CEM-213env1, -env4, and -env7, were isolated. These clones expressed high levels of surface gp41 and gp120, as demonstrated by flow cytometry with anti-HIV env monoclonal antibodies. Processing and function of env was shown by induction of syncytia with CD4-expressing HeLa cells and by immunoblot analysis. The env expression resulted in specific down-regulation of surface CD4 levels, supporting the role of HIV env, in CD4 modulation. Furthermore, serum samples from nine of nine HIV-1-infected individuals bound specifically to the env-expressing transfectants, substantiating the presence of conserved antigenic determinants. These sera also mediated antibody-dependent cellular cytotoxicity (ADCC) of the env-expressing cell lines. The env-expressing cell lines provide a relevant, safe, and practical model for qualitative and quantitative analysis of humoral and cellular immune responses and their role in HIV-1 pathogenesis and therapy.

Original languageEnglish (US)
Pages (from-to)117-126
Number of pages10
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume13
Issue number2
DOIs
StatePublished - Jan 1 1996

Keywords

  • Antibody-dependent cellular cytotoxicity
  • CD4 modulation
  • HIV envelope
  • Syncytia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

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