Developmental patterns of serum 3α-androstanediol glucuronide

H. L. Rittner, Phillip Lee, W. F. Blum, H. G. Doerr, J. Steiss, J. Kreuder, W. Rascher, W. Kiess

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

3α-androstanediol glucuronide (3αdiolG) is a marker of peripheral tissue androgen metabolism. There are no previous data regarding complete paediatric reference ranges for 3αdiolG. In order to obtain reference values for 3αdiolG we have measured serum levels of 3αdiolG in 283 healthy children and adolescents, 146 boys and 137 girls, age 1 month to 20 years and 28 adults. A non-extraction, solid phase radioimmunoassay employing a polyclonal antiserum that is specific for 3αdiolG was used to measure serum 3αdiolG levels (intra assay variation 5.1-10.1%, inter assay variation 2.7-9.0%). There was a strong sex and age dependence (r = 0.8; p < 0.0001) of 3αdiolG levels throughout childhood and adolescence with males showing significantly higher levels of the androgen than females (p < 0.05). 3αdiolG serum levels (nmol/l ± SD) correlated significantly with pubertal stage (p < 0.01). Interestingly, in 35 children with CAH serum 3αdiolG levels correlated well with clinical and metabolic status, i.e. 170HP serum levels. In summary, we have established percentile curves for 3αdiolG levels in healthy children and adolescents. We hypothesize that on the basis of our reference values the single measurement of serum 3αdiolG could serve as a means to determine androgen status in children with disorders of puberty and sexual development.

Original languageEnglish (US)
Pages (from-to)138-143
Number of pages6
JournalJournal of Endocrinological Investigation
Volume20
Issue number3
StatePublished - Mar 1997
Externally publishedYes

Fingerprint

Glucuronides
Serum
Androgens
Reference Values
Disorders of Sex Development
Puberty
Radioimmunoassay
Immune Sera
Pediatrics

Keywords

  • 17-hydroxyprogesterone
  • 3α-androstanediol
  • 3α-androstanediol glucuronide
  • Adrenal
  • Androgens
  • Congenital adrenal hyperplasia
  • Puberty

ASJC Scopus subject areas

  • Endocrinology

Cite this

Rittner, H. L., Lee, P., Blum, W. F., Doerr, H. G., Steiss, J., Kreuder, J., ... Kiess, W. (1997). Developmental patterns of serum 3α-androstanediol glucuronide. Journal of Endocrinological Investigation, 20(3), 138-143.

Developmental patterns of serum 3α-androstanediol glucuronide. / Rittner, H. L.; Lee, Phillip; Blum, W. F.; Doerr, H. G.; Steiss, J.; Kreuder, J.; Rascher, W.; Kiess, W.

In: Journal of Endocrinological Investigation, Vol. 20, No. 3, 03.1997, p. 138-143.

Research output: Contribution to journalArticle

Rittner, HL, Lee, P, Blum, WF, Doerr, HG, Steiss, J, Kreuder, J, Rascher, W & Kiess, W 1997, 'Developmental patterns of serum 3α-androstanediol glucuronide', Journal of Endocrinological Investigation, vol. 20, no. 3, pp. 138-143.
Rittner HL, Lee P, Blum WF, Doerr HG, Steiss J, Kreuder J et al. Developmental patterns of serum 3α-androstanediol glucuronide. Journal of Endocrinological Investigation. 1997 Mar;20(3):138-143.
Rittner, H. L. ; Lee, Phillip ; Blum, W. F. ; Doerr, H. G. ; Steiss, J. ; Kreuder, J. ; Rascher, W. ; Kiess, W. / Developmental patterns of serum 3α-androstanediol glucuronide. In: Journal of Endocrinological Investigation. 1997 ; Vol. 20, No. 3. pp. 138-143.
@article{77287eb8a3434254af90c1ed79abd9d4,
title = "Developmental patterns of serum 3α-androstanediol glucuronide",
abstract = "3α-androstanediol glucuronide (3αdiolG) is a marker of peripheral tissue androgen metabolism. There are no previous data regarding complete paediatric reference ranges for 3αdiolG. In order to obtain reference values for 3αdiolG we have measured serum levels of 3αdiolG in 283 healthy children and adolescents, 146 boys and 137 girls, age 1 month to 20 years and 28 adults. A non-extraction, solid phase radioimmunoassay employing a polyclonal antiserum that is specific for 3αdiolG was used to measure serum 3αdiolG levels (intra assay variation 5.1-10.1{\%}, inter assay variation 2.7-9.0{\%}). There was a strong sex and age dependence (r = 0.8; p < 0.0001) of 3αdiolG levels throughout childhood and adolescence with males showing significantly higher levels of the androgen than females (p < 0.05). 3αdiolG serum levels (nmol/l ± SD) correlated significantly with pubertal stage (p < 0.01). Interestingly, in 35 children with CAH serum 3αdiolG levels correlated well with clinical and metabolic status, i.e. 170HP serum levels. In summary, we have established percentile curves for 3αdiolG levels in healthy children and adolescents. We hypothesize that on the basis of our reference values the single measurement of serum 3αdiolG could serve as a means to determine androgen status in children with disorders of puberty and sexual development.",
keywords = "17-hydroxyprogesterone, 3α-androstanediol, 3α-androstanediol glucuronide, Adrenal, Androgens, Congenital adrenal hyperplasia, Puberty",
author = "Rittner, {H. L.} and Phillip Lee and Blum, {W. F.} and Doerr, {H. G.} and J. Steiss and J. Kreuder and W. Rascher and W. Kiess",
year = "1997",
month = "3",
language = "English (US)",
volume = "20",
pages = "138--143",
journal = "Journal of Endocrinological Investigation",
issn = "0391-4097",
publisher = "Editrice Kurtis s.r.l.",
number = "3",

}

TY - JOUR

T1 - Developmental patterns of serum 3α-androstanediol glucuronide

AU - Rittner, H. L.

AU - Lee, Phillip

AU - Blum, W. F.

AU - Doerr, H. G.

AU - Steiss, J.

AU - Kreuder, J.

AU - Rascher, W.

AU - Kiess, W.

PY - 1997/3

Y1 - 1997/3

N2 - 3α-androstanediol glucuronide (3αdiolG) is a marker of peripheral tissue androgen metabolism. There are no previous data regarding complete paediatric reference ranges for 3αdiolG. In order to obtain reference values for 3αdiolG we have measured serum levels of 3αdiolG in 283 healthy children and adolescents, 146 boys and 137 girls, age 1 month to 20 years and 28 adults. A non-extraction, solid phase radioimmunoassay employing a polyclonal antiserum that is specific for 3αdiolG was used to measure serum 3αdiolG levels (intra assay variation 5.1-10.1%, inter assay variation 2.7-9.0%). There was a strong sex and age dependence (r = 0.8; p < 0.0001) of 3αdiolG levels throughout childhood and adolescence with males showing significantly higher levels of the androgen than females (p < 0.05). 3αdiolG serum levels (nmol/l ± SD) correlated significantly with pubertal stage (p < 0.01). Interestingly, in 35 children with CAH serum 3αdiolG levels correlated well with clinical and metabolic status, i.e. 170HP serum levels. In summary, we have established percentile curves for 3αdiolG levels in healthy children and adolescents. We hypothesize that on the basis of our reference values the single measurement of serum 3αdiolG could serve as a means to determine androgen status in children with disorders of puberty and sexual development.

AB - 3α-androstanediol glucuronide (3αdiolG) is a marker of peripheral tissue androgen metabolism. There are no previous data regarding complete paediatric reference ranges for 3αdiolG. In order to obtain reference values for 3αdiolG we have measured serum levels of 3αdiolG in 283 healthy children and adolescents, 146 boys and 137 girls, age 1 month to 20 years and 28 adults. A non-extraction, solid phase radioimmunoassay employing a polyclonal antiserum that is specific for 3αdiolG was used to measure serum 3αdiolG levels (intra assay variation 5.1-10.1%, inter assay variation 2.7-9.0%). There was a strong sex and age dependence (r = 0.8; p < 0.0001) of 3αdiolG levels throughout childhood and adolescence with males showing significantly higher levels of the androgen than females (p < 0.05). 3αdiolG serum levels (nmol/l ± SD) correlated significantly with pubertal stage (p < 0.01). Interestingly, in 35 children with CAH serum 3αdiolG levels correlated well with clinical and metabolic status, i.e. 170HP serum levels. In summary, we have established percentile curves for 3αdiolG levels in healthy children and adolescents. We hypothesize that on the basis of our reference values the single measurement of serum 3αdiolG could serve as a means to determine androgen status in children with disorders of puberty and sexual development.

KW - 17-hydroxyprogesterone

KW - 3α-androstanediol

KW - 3α-androstanediol glucuronide

KW - Adrenal

KW - Androgens

KW - Congenital adrenal hyperplasia

KW - Puberty

UR - http://www.scopus.com/inward/record.url?scp=0030986576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030986576&partnerID=8YFLogxK

M3 - Article

C2 - 9186820

AN - SCOPUS:0030986576

VL - 20

SP - 138

EP - 143

JO - Journal of Endocrinological Investigation

JF - Journal of Endocrinological Investigation

SN - 0391-4097

IS - 3

ER -