Developmental toxicity assay using high content screening of zebrafish embryos

Susan Lantz-Mcpeak, Xiaoqing Guo, Elvis Cuevas, Melanie Dumas, Glenn D. Newport, Syed F. Ali, Merle G. Paule, Jyotshna Kanungo

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Typically, time-consuming standard toxicological assays using the zebrafish (Danio rerio) embryo model evaluate mortality and teratogenicity after exposure during the first 2days post-fertilization. Here we describe an automated image-based high content screening (HCS) assay to identify the teratogenic/embryotoxic potential of compounds in zebrafish embryos in vivo. Automated image acquisition was performed using a high content microscope system. Further automated analysis of embryo length, as a statistically quantifiable endpoint of toxicity, was performed on images post-acquisition. The biological effects of ethanol, nicotine, ketamine, caffeine, dimethyl sulfoxide and temperature on zebrafish embryos were assessed. This automated developmental toxicity assay, based on a growth-retardation endpoint should be suitable for evaluating the effects of potential teratogens and developmental toxicants in a high throughput manner. This approach can significantly expedite the screening of potential teratogens and developmental toxicants, thereby improving the current risk assessment process by decreasing analysis time and required resources. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

Original languageEnglish (US)
Pages (from-to)261-272
Number of pages12
JournalJournal of Applied Toxicology
Volume35
Issue number3
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Keywords

  • Automated imaging
  • Ethanol
  • High content screening
  • Integrated morphometric analysis
  • Ketamine
  • Nicotine
  • Zebrafish embryo

ASJC Scopus subject areas

  • Toxicology

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