Dhr6, a Drosophila homolog of the yeast DNA-repair gene RAD6

Marcel Koken, Paul Reynolds, Dirk Bootsma, Jan Hoeijmakers, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The RAD6 gene of the yeast Saccharomyces cerevisiae is required for DNA repair, for DNA damage-induced mutagenesis, and for sporulation, and it encodes a ubiquitin-conjugating enzyme. We have cloned the RAD6 homolog from Drosophila melanogaster and find that its encoded protein displays a very high degree of identity in amino acid sequence with the homologous RAD6 proteins from the two divergent yeasts, S. cerevisiae and Schizosaccharomyces pombe, and from human. Genetic complementation studies indicate that the Drosophila RAD6 homolog can functionally substitute for the S. cerevisiae RAD6 gene in its DNA-repair and UV-mutagenesis functions but cannot substitute in sporulation. The high degree of structural and functional conservation of RAD6 in eukaryotic evolution suggests that the various protein components involved in RAD6-dependent DNA repair and mutagenesis functions have also been conserved.

Original languageEnglish (US)
Pages (from-to)3832-3836
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number9
StatePublished - 1991
Externally publishedYes

Fingerprint

Mutagenesis
DNA Repair
Drosophila
Saccharomyces cerevisiae
Schizosaccharomyces
Yeasts
Ubiquitin-Conjugating Enzymes
Genes
Amino Acid Sequence Homology
Proteins
Drosophila melanogaster
DNA Damage

Keywords

  • DNA damage
  • E2 enzyme
  • Sporulation
  • Ubiquitin conjugation
  • UV mutagenesis

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Dhr6, a Drosophila homolog of the yeast DNA-repair gene RAD6. / Koken, Marcel; Reynolds, Paul; Bootsma, Dirk; Hoeijmakers, Jan; Prakash, Satya; Prakash, Louise.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 9, 1991, p. 3832-3836.

Research output: Contribution to journalArticle

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