Diabetes impairs sciatic nerve hyperemia induced by surgical trauma: Implications for diabetic neuropathy

Yasuo Ido, Kathy Chang, Wanda LeJeune, Ronald Tilton, William W. Monafo, Joseph R. Williamson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The most widely used methods to assess nerve blood flow in diabetic rats are hydrogen clearance polarography and laser Doppler flowmetry, techniques requiring surgical exposure of the nerve. In these experiments, we examined the hypothesis that the trauma of surgical exposure introduces an important and hitherto largely unrecognized variable that could account for discordant reports on nerve blood flew changes induced by diabetes. We used the noninvasive (for sciatic nerve) reference sample microsphere method to quantify sciatic nerve blood flow in unexposed vs. surgically exposed nerves in rats with streptozotocin-induced diabetes (at different temperatures and after curarization) and in unexposed vs. surgically exposed nerves in galactose-fed rats. Baseline resting blood flow in unexposed nerves in both animal models of diabetes was either normal or increased (but was decreased in diabetic rats given d-tubocurarine). Furthermore, the normal brisk hyperemic nerve blood flow response to the minimal trauma associated with surgical exposure of the nerve was markedly impaired in diabetic and in galactose-fed rats. Normalization of the blood flow response to trauma in galactose-fed rats by an aldose reductase inhibitor suggests that the impairment is linked to increased polyol pathway metabolism. These findings 1) confirm our previous findings that sciatic nerve blood flow in diabetic rats is increased or unchanged in unexposed nerves, while also confirming reports that in surgically exposed nerves blood flew is higher in control than in diabetic rats, and 2) indicate that blood flows in surgically exposed nerves are largely a measure of vascular responses to injury rather than (patho)physiological blood flow in undisturbed nerves.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume273
Issue number1 36-1
StatePublished - Jul 1997
Externally publishedYes

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Diabetic Neuropathies
Hyperemia
Sciatic Nerve
Medical problems
Blood
Rats
Wounds and Injuries
Galactose
Polarography
Polarographic analysis
Tubocurarine
Aldehyde Reductase
Laser-Doppler Flowmetry
Experimental Diabetes Mellitus
Streptozocin
Microspheres
Metabolism
Blood Vessels
Hydrogen
Animals

Keywords

  • Acute inflammation
  • Curare

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Diabetes impairs sciatic nerve hyperemia induced by surgical trauma : Implications for diabetic neuropathy. / Ido, Yasuo; Chang, Kathy; LeJeune, Wanda; Tilton, Ronald; Monafo, William W.; Williamson, Joseph R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 273, No. 1 36-1, 07.1997.

Research output: Contribution to journalArticle

Ido, Yasuo ; Chang, Kathy ; LeJeune, Wanda ; Tilton, Ronald ; Monafo, William W. ; Williamson, Joseph R. / Diabetes impairs sciatic nerve hyperemia induced by surgical trauma : Implications for diabetic neuropathy. In: American Journal of Physiology - Endocrinology and Metabolism. 1997 ; Vol. 273, No. 1 36-1.
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