Diabetes-induced apoptosis in rat kidney

Weiping Zhang, Peeyush Khanna, Lillian L. Chan, Gerald Campbell, Naseem Ansari

Research output: Contribution to journalArticle

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Abstract

Oxidative stress has been suggested to play a crucial role in the pathogenesis of diabetic complications including nephropathy. However, the exact mechanism of diabetic nephropathy is still not clearly understood. Since oxidative stress is known to be a major component in the induction of apoptosis, we investigated the occurrence of apoptosis in diabetic rat kidney. The status of oxidative stress was determined as thiobarbituric acid reactive substances (TBARS). The TBARS in the control and diabetic rat kidney were 2.00 ± 0.963 and 3.83 ± 0.715 μmol/mg protein, respectively (P < 0.05). Apoptosis was determined by evaluating the DNA fragmentation using an enzyme-linked immunoassay and in situ end labeling. DNA fragmentation increased approximately fourfold in diabetic rat kidney compared to the normal kidney (P < 0.05). Apoptag in situ labeling displayed negligible apoptosis in nondiabetic kidney while significant areas of apoptosis were observed in diabetic kidney. Our results suggest that increased oxidative stress in diabetic kidney could induce apoptosis, which may contribute to the development of diabetic nephropathy.

Original languageEnglish (US)
Pages (from-to)58-62
Number of pages5
JournalBiochemical and Molecular Medicine
Volume61
Issue number1
DOIs
StatePublished - Jun 1997

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Medical problems
Rats
Oxidative stress
Apoptosis
Kidney
Oxidative Stress
Thiobarbituric Acid Reactive Substances
Diabetic Nephropathies
Labeling
DNA Fragmentation
DNA
Diabetes Complications
Immunoenzyme Techniques
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Diabetes-induced apoptosis in rat kidney. / Zhang, Weiping; Khanna, Peeyush; Chan, Lillian L.; Campbell, Gerald; Ansari, Naseem.

In: Biochemical and Molecular Medicine, Vol. 61, No. 1, 06.1997, p. 58-62.

Research output: Contribution to journalArticle

Zhang, Weiping ; Khanna, Peeyush ; Chan, Lillian L. ; Campbell, Gerald ; Ansari, Naseem. / Diabetes-induced apoptosis in rat kidney. In: Biochemical and Molecular Medicine. 1997 ; Vol. 61, No. 1. pp. 58-62.
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