TY - JOUR
T1 - Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay
AU - Rotbart, H. A.
AU - Sawyer, M. H.
AU - Fast, S.
AU - Lewinski, C.
AU - Murphy, N.
AU - Keyser, E. F.
AU - Spadoro, J.
AU - Kao, S. Y.
AU - Loeffelholz, M.
PY - 1994
Y1 - 1994
N2 - A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.
AB - A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0028129104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028129104&partnerID=8YFLogxK
U2 - 10.1128/jcm.32.10.2590-2592.1994
DO - 10.1128/jcm.32.10.2590-2592.1994
M3 - Comment/debate
C2 - 7814507
AN - SCOPUS:0028129104
SN - 0095-1137
VL - 32
SP - 2590
EP - 2592
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 10
ER -