Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

H. A. Rotbart, M. H. Sawyer, S. Fast, C. Lewinski, N. Murphy, E. F. Keyser, J. Spadoro, S. Y. Kao, M. Loeffelholz

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

Original languageEnglish (US)
Pages (from-to)2590-2592
Number of pages3
JournalJournal of Clinical Microbiology
Volume32
Issue number10
StatePublished - 1994
Externally publishedYes

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Uracil-DNA Glycosidase
Meningitis
Reverse Transcription
Sensitivity and Specificity
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Rotbart, H. A., Sawyer, M. H., Fast, S., Lewinski, C., Murphy, N., Keyser, E. F., ... Loeffelholz, M. (1994). Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. Journal of Clinical Microbiology, 32(10), 2590-2592.

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. / Rotbart, H. A.; Sawyer, M. H.; Fast, S.; Lewinski, C.; Murphy, N.; Keyser, E. F.; Spadoro, J.; Kao, S. Y.; Loeffelholz, M.

In: Journal of Clinical Microbiology, Vol. 32, No. 10, 1994, p. 2590-2592.

Research output: Contribution to journalArticle

Rotbart, HA, Sawyer, MH, Fast, S, Lewinski, C, Murphy, N, Keyser, EF, Spadoro, J, Kao, SY & Loeffelholz, M 1994, 'Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay', Journal of Clinical Microbiology, vol. 32, no. 10, pp. 2590-2592.
Rotbart HA, Sawyer MH, Fast S, Lewinski C, Murphy N, Keyser EF et al. Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. Journal of Clinical Microbiology. 1994;32(10):2590-2592.
Rotbart, H. A. ; Sawyer, M. H. ; Fast, S. ; Lewinski, C. ; Murphy, N. ; Keyser, E. F. ; Spadoro, J. ; Kao, S. Y. ; Loeffelholz, M. / Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. In: Journal of Clinical Microbiology. 1994 ; Vol. 32, No. 10. pp. 2590-2592.
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