Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

H. A. Rotbart, M. H. Sawyer, S. Fast, C. Lewinski, N. Murphy, E. F. Keyser, J. Spadoro, S. Y. Kao, M. Loeffelholz

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181 Scopus citations

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

Original languageEnglish (US)
Pages (from-to)2590-2592
Number of pages3
JournalJournal of Clinical Microbiology
Volume32
Issue number10
StatePublished - Jan 1 1994

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ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Rotbart, H. A., Sawyer, M. H., Fast, S., Lewinski, C., Murphy, N., Keyser, E. F., Spadoro, J., Kao, S. Y., & Loeffelholz, M. (1994). Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. Journal of Clinical Microbiology, 32(10), 2590-2592.