Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

H. A. Rotbart; M. H. Sawyer; S. Fast; C. Lewinski; N. Murphy; E. F. Keyser; J. Spadoro; S. Y. Kao; M. Loeffelholz

doi:10.1128/jcm.32.10.2590-2592.1994

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

H. A. Rotbart
, M. H. Sawyer
, S. Fast
, C. Lewinski
, N. Murphy
, E. F. Keyser
, J. Spadoro
, S. Y. Kao
, M. Loeffelholz

Research output: Contribution to journal › Comment/debate › peer-review

204 Scopus citations

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

Original language	English (US)
Pages (from-to)	2590-2592
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	32
Issue number	10
DOIs	https://doi.org/10.1128/jcm.32.10.2590-2592.1994
State	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.32.10.2590-2592.1994

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title = "Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay",

abstract = "A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4\%, respectively.",

author = "Rotbart, \{H. A.\} and Sawyer, \{M. H.\} and S. Fast and C. Lewinski and N. Murphy and Keyser, \{E. F.\} and J. Spadoro and Kao, \{S. Y.\} and M. Loeffelholz",

year = "1994",

doi = "10.1128/jcm.32.10.2590-2592.1994",

language = "English (US)",

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pages = "2590--2592",

journal = "Journal of Clinical Microbiology",

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TY - JOUR

T1 - Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

AU - Rotbart, H. A.

AU - Sawyer, M. H.

AU - Fast, S.

AU - Lewinski, C.

AU - Murphy, N.

AU - Keyser, E. F.

AU - Spadoro, J.

AU - Kao, S. Y.

AU - Loeffelholz, M.

PY - 1994

Y1 - 1994

N2 - A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

AB - A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

UR - https://www.scopus.com/pages/publications/0028129104

UR - https://www.scopus.com/pages/publications/0028129104#tab=citedBy

U2 - 10.1128/jcm.32.10.2590-2592.1994

DO - 10.1128/jcm.32.10.2590-2592.1994

M3 - Comment/debate

C2 - 7814507

AN - SCOPUS:0028129104

SN - 0095-1137

VL - 32

SP - 2590

EP - 2592

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 10

ER -

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

Abstract

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