Didymin by suppressing NF-κB activation prevents VEGF-induced angiogenesis in vitro and in vivo

Kirtikar Shukla, Himangshu Sonowal, Ashish Saxena, Kota Ramana

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Although didymin, a dietary flavonoid glycoside from citrus fruits, known to be a potent antioxidant with anti-cancer activities, its role in angiogenesis is not known. In this study, we examined the effect of didymin on VEGF-induced angiogenesis in vitro and in vivo models. Our results suggest that treatment of human umbilical vein endothelial cell (HUVECs) with didymin significantly prevented the VEGF-induced cell proliferation, migration, and invasion. Further, didymin significantly prevented the VEGF-induced endothelial tube formation in culture. Didymin also attenuated the VEGF-induced generation of ROS, activation of NF-κB and the expression of adhesion molecules such as VCAM-1, ICAM-1, and E-selectin in HUVECs. Further, didymin also prevented the VEGF-induced microvessel sprouting in ex vivo mouse aortic rings. Most importantly, didymin significantly prevented the invasion of endothelial cells and formation of blood capillary-like structures in Matrigel plug model of angiogenesis in mice. Thus, our results suggest a novel antiangiogenic efficacy of didymin in addition to its reported anti-cancer properties, which warrant further development of this agent for cancer therapy.

Original languageEnglish (US)
JournalVascular Pharmacology
DOIs
StateAccepted/In press - Jan 1 2019

Keywords

  • Angiogenesis
  • Didymin
  • HUVEC
  • Neovascularization
  • VEGF

ASJC Scopus subject areas

  • Physiology
  • Molecular Medicine
  • Pharmacology

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