Didymin prevents hyperglycemia-induced human umbilical endothelial cells dysfunction and death

Kirtikar Shukla, Himangshu Sonowal, Ashish Saxena, Kota Ramana

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9 Scopus citations

Abstract

Although didymin, a flavonoid-O-glycosides compound naturally found in the citrus fruits, has been reported to be a potent anticancer agent in the prevention of various cancers, its role in the prevention of cardiovascular complications is unclear. Most importantly, its effect in the prevention of endothelial dysfunction, a pathological process involved in the atherogenesis, is unknown. We have examined the efficacy of didymin in preventing the high glucose (HG; 25 mM)-induced human umbilical vein endothelial cells (HUVECs) dysfunction. Our results indicate that incubation of HUVECs with HG resulted in the loss of cell viability, and pre-incubation of didymin prevented it. Further, didymin prevented the HG-induced generation of reactive oxygen species (ROS) as well as lipid peroxidation product, malondialdehyde. Pretreatment of HUVECs with didymin also prevented the HG-induced decrease in eNOS and increase in iNOS expressions. Further, didymin prevented the HG-induced monocytes cell adhesion to endothelial cells, expressions of ICAM-1 and VCAM-1 and activation of NF-κB. Didymin also prevented the release of various inflammatory cytokines and chemokines in HG-treated HUVECs. In conclusion, our results demonstrate that didymin with its anti-oxidative and anti-inflammatory actions prevents hyperglycemia-induced endothelial dysfunction and death. Thus, it could be developed as a potential natural therapeutic agent for the prevention of cardiovascular complications in diabetes.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalBiochemical Pharmacology
Volume152
DOIs
StatePublished - Jun 1 2018

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Keywords

  • Didymin
  • Endothelial cells
  • Hyperglycemia
  • Inflammation
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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