TY - JOUR
T1 - Diet-induced obesity causes hypothalamic neurochemistry alterations in Swiss mice
AU - de Bona Schraiber, Rosiane
AU - de Mello, Aline Haas
AU - Garcez, Michelle Lima
AU - de Bem Silveira, Gustavo
AU - Zacaron, Rubya Pereira
AU - de Souza Goldim, Mariana Pereira
AU - Budni, Josiane
AU - Silveira, Paulo Cesar Lock
AU - Petronilho, Fabrícia
AU - Ferreira, Gabriela Kozuchovski
AU - Rezin, Gislaine Tezza
N1 - Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019/4/15
Y1 - 2019/4/15
N2 - The aim of this study was to assess inflammatory parameters, oxidative stress and energy metabolism in the hypothalamus of diet-induced obese mice. Male Swiss mice were divided into two study groups: control group and obese group. The animals in the control group were fed a diet with adequate amounts of macronutrients (normal-lipid diet), whereas the animals in the obese group were fed a high-fat diet to induce obesity. Obesity induction lasted 10 weeks, at the end of this period the disease model was validated in animals. The animals in the obese group had higher calorie consumption, higher body weight and higher weight of mesenteric fat compared to control group. Obesity showed an increase in levels of interleukin 1β and decreased levels of interleukin 10 in the hypothalamus. Furthermore, increased lipid peroxidation and protein carbonylation, and decreased level of glutathione in the hypothalamus of obese animals. However, there was no statistically significant difference in the activity of antioxidant enzymes, superoxide dismutase and catalase. The obese group had lower activity of complex I, II and IV of the mitochondrial respiratory chain, as well as lower activity of creatine kinase in the hypothalamus as compared to the control group. Thus, the results from this study showed changes in inflammatory markers, and dysregulation of metabolic enzymes in the pathophysiology of obesity.
AB - The aim of this study was to assess inflammatory parameters, oxidative stress and energy metabolism in the hypothalamus of diet-induced obese mice. Male Swiss mice were divided into two study groups: control group and obese group. The animals in the control group were fed a diet with adequate amounts of macronutrients (normal-lipid diet), whereas the animals in the obese group were fed a high-fat diet to induce obesity. Obesity induction lasted 10 weeks, at the end of this period the disease model was validated in animals. The animals in the obese group had higher calorie consumption, higher body weight and higher weight of mesenteric fat compared to control group. Obesity showed an increase in levels of interleukin 1β and decreased levels of interleukin 10 in the hypothalamus. Furthermore, increased lipid peroxidation and protein carbonylation, and decreased level of glutathione in the hypothalamus of obese animals. However, there was no statistically significant difference in the activity of antioxidant enzymes, superoxide dismutase and catalase. The obese group had lower activity of complex I, II and IV of the mitochondrial respiratory chain, as well as lower activity of creatine kinase in the hypothalamus as compared to the control group. Thus, the results from this study showed changes in inflammatory markers, and dysregulation of metabolic enzymes in the pathophysiology of obesity.
KW - Energy metabolism
KW - Hypothalamus
KW - Inflammation
KW - Obesity
KW - Oxidative stress
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U2 - 10.1007/s11011-018-0337-9
DO - 10.1007/s11011-018-0337-9
M3 - Article
C2 - 30635861
AN - SCOPUS:85059894802
SN - 0885-7490
VL - 34
SP - 565
EP - 573
JO - Metabolic brain disease
JF - Metabolic brain disease
IS - 2
ER -