Diet-induced obesity causes hypothalamic neurochemistry alterations in Swiss mice

Rosiane de Bona Schraiber, Aline Haas de Mello, Michelle Lima Garcez, Gustavo de Bem Silveira, Rubya Pereira Zacaron, Mariana Pereira de Souza Goldim, Josiane Budni, Paulo Cesar Lock Silveira, Fabrícia Petronilho, Gabriela Kozuchovski Ferreira, Gislaine Tezza Rezin

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The aim of this study was to assess inflammatory parameters, oxidative stress and energy metabolism in the hypothalamus of diet-induced obese mice. Male Swiss mice were divided into two study groups: control group and obese group. The animals in the control group were fed a diet with adequate amounts of macronutrients (normal-lipid diet), whereas the animals in the obese group were fed a high-fat diet to induce obesity. Obesity induction lasted 10 weeks, at the end of this period the disease model was validated in animals. The animals in the obese group had higher calorie consumption, higher body weight and higher weight of mesenteric fat compared to control group. Obesity showed an increase in levels of interleukin 1β and decreased levels of interleukin 10 in the hypothalamus. Furthermore, increased lipid peroxidation and protein carbonylation, and decreased level of glutathione in the hypothalamus of obese animals. However, there was no statistically significant difference in the activity of antioxidant enzymes, superoxide dismutase and catalase. The obese group had lower activity of complex I, II and IV of the mitochondrial respiratory chain, as well as lower activity of creatine kinase in the hypothalamus as compared to the control group. Thus, the results from this study showed changes in inflammatory markers, and dysregulation of metabolic enzymes in the pathophysiology of obesity.

Original languageEnglish (US)
Pages (from-to)565-573
Number of pages9
JournalMetabolic brain disease
Issue number2
StatePublished - Apr 15 2019
Externally publishedYes


  • Energy metabolism
  • Hypothalamus
  • Inflammation
  • Obesity
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Neurology
  • Cellular and Molecular Neuroscience


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