Dietary arginine uptake by the splanchnic region in adult humans

Leticia Castillo, T. E. Chapman, Y. M. Yu, A. Ajami, J. F. Burke, V. R. Young

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

To determine the uptake of dietary arginine and leucine by the splanchnic region, two experiments were carried out, each involving four healthy young adult men who received a diet supplying 1 g protein · kg-1 · day-1 for 7 and 10 days before conducting a primed constant tracer infusion protocol. In study 1, subjects received for 8 h (3-h fast; 5-h fed state, achieved by a constant intragastric infusion of the diet formula) L-[5,5-2H2; guanidino- 15N2]arginine ([M4]Arg), L-[guanidino-13C]arginine ([13C]Arg), and L- [5,5,5-2H3]leucine ([2H3]Leu) simultaneously by an intragastric infusion on day 7 and a repeat of this protocol on day 10 except with tracer administration given by vein. Plasma arginine fluxes were essentially the same for the two arginine tracers but differed significantly with route of administration. In study 2 the subjects received on day 7 a constant intravenous infusion of [13C]Arg and [2H3]Leu and a simultaneous intragastric infusion of [M4]Arg and [1-13C]leucine. On day 10 the routes of administration of these tracer pairs were reversed. During the fed state in study 1, splanchnic uptake of dietary arginine was 31 ± 10 and 34 ± 8%, based on the [13C]Arg and [M4]Arg tracers, respectively, and it was significantly higher (P < 0.01) than for leucine, which was 10 ± 6%. In study 2, splanchnic uptake of dietary arginine, estimated from a series of tracer-protocol combinations for the fed state, was ~38% compared with a lower (P < 0.01) value of ~15% for leucine.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume265
Issue number4 28-4
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Viscera
Arginine
Leucine
Formulated Food
Intravenous Infusions
Young Adult
Veins
Diet

Keywords

  • first pass
  • leucine
  • splanchnic uptake

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Castillo, L., Chapman, T. E., Yu, Y. M., Ajami, A., Burke, J. F., & Young, V. R. (1993). Dietary arginine uptake by the splanchnic region in adult humans. American Journal of Physiology - Endocrinology and Metabolism, 265(4 28-4).

Dietary arginine uptake by the splanchnic region in adult humans. / Castillo, Leticia; Chapman, T. E.; Yu, Y. M.; Ajami, A.; Burke, J. F.; Young, V. R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 265, No. 4 28-4, 01.01.1993.

Research output: Contribution to journalArticle

Castillo, Leticia ; Chapman, T. E. ; Yu, Y. M. ; Ajami, A. ; Burke, J. F. ; Young, V. R. / Dietary arginine uptake by the splanchnic region in adult humans. In: American Journal of Physiology - Endocrinology and Metabolism. 1993 ; Vol. 265, No. 4 28-4.
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