Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults

Madonna M. Mamerow, Joni A. Mettler, Kirk L. English, Shanon L. Casperson, Emily Arentson-Lantz, Melinda Sheffield-Moore, Donald K. Layman, Douglas Paddon-Jones

Research output: Contribution to journalArticle

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Abstract

The RDA for protein describes the quantity that should be consumed daily to meet population needs and to prevent deficiency. Protein consumption in many countries exceeds the RDA; however, intake is often skewed toward the evening meal, whereas breakfast is typically carbohydrate rich and low in protein. We examined the effects of protein distribution on 24-h skeletal muscle protein synthesis in healthy adult men and women (n = 8; age: 36.9 ± 3.1 y; BMI: 25.7 ± 0.8 kg/m2). By using a 7-d crossover feeding design with a 30-d washout period, we measured changes in muscle protein synthesis in response to isoenergetic and isonitrogenous diets with protein at breakfast, lunch, and dinner distributed evenly (EVEN; 31.5 ± 1.3, 29.9 ± 1.6, and 32.7 ± 1.6 g protein, respectively) or skewed (SKEW; 10.7 ± 0.8, 16.0 ± 0.5, and 63.4 ± 3.7 g protein, respectively). Over 24-h periods on days 1 and 7, venous blood samples and vastus lateralis muscle biopsy samples were obtained during primed (2.0 μmol/kg) constant infusion [0.06 μmol/(kg-min)] of L-[ring-13C6] phenylalanine. The 24-h mixed muscle protein fractional synthesis rate was 25% higher in the EVEN (0.075 ± 0.006%/h) vs. the SKEW (0.056 ± 0.006%/h) protein distribution groups (P = 0.003). This pattern was maintained after 7 d of habituation to each diet (EVEN vs. SKEW: 0.077 ± 0.006 vs. 0.056 ± 0.006%/h; P = 0.001). The consumption of a moderate amount of protein at each meal stimulated 24-h muscle protein synthesis more effectively than skewing protein intake toward the evening meal.

Original languageEnglish (US)
Pages (from-to)876-880
Number of pages5
JournalJournal of Nutrition
Volume144
Issue number6
DOIs
StatePublished - 2014

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Dietary Proteins
Muscle Proteins
Proteins
Meals
Breakfast
Diet
Lunch
Quadriceps Muscle
Phenylalanine
Cross-Over Studies
Skeletal Muscle
Carbohydrates
Biopsy
Muscles

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Mamerow, M. M., Mettler, J. A., English, K. L., Casperson, S. L., Arentson-Lantz, E., Sheffield-Moore, M., ... Paddon-Jones, D. (2014). Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults. Journal of Nutrition, 144(6), 876-880. https://doi.org/10.3945/jn.113.185280

Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults. / Mamerow, Madonna M.; Mettler, Joni A.; English, Kirk L.; Casperson, Shanon L.; Arentson-Lantz, Emily; Sheffield-Moore, Melinda; Layman, Donald K.; Paddon-Jones, Douglas.

In: Journal of Nutrition, Vol. 144, No. 6, 2014, p. 876-880.

Research output: Contribution to journalArticle

Mamerow, MM, Mettler, JA, English, KL, Casperson, SL, Arentson-Lantz, E, Sheffield-Moore, M, Layman, DK & Paddon-Jones, D 2014, 'Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults', Journal of Nutrition, vol. 144, no. 6, pp. 876-880. https://doi.org/10.3945/jn.113.185280
Mamerow MM, Mettler JA, English KL, Casperson SL, Arentson-Lantz E, Sheffield-Moore M et al. Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults. Journal of Nutrition. 2014;144(6):876-880. https://doi.org/10.3945/jn.113.185280
Mamerow, Madonna M. ; Mettler, Joni A. ; English, Kirk L. ; Casperson, Shanon L. ; Arentson-Lantz, Emily ; Sheffield-Moore, Melinda ; Layman, Donald K. ; Paddon-Jones, Douglas. / Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults. In: Journal of Nutrition. 2014 ; Vol. 144, No. 6. pp. 876-880.
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