TY - JOUR
T1 - Differences in Genetic Diversity of Mammalian Tick-Borne Flaviviruses
AU - Carpio, Kassandra L.
AU - Thompson, Jill K.
AU - Widen, Steven G.
AU - Smith, Jennifer K.
AU - Juelich, Terry L.
AU - Clements, David E.
AU - Freiberg, Alexander N.
AU - Barrett, Alan D.T.
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/2
Y1 - 2023/2
N2 - The genetic diversities of mammalian tick-borne flaviviruses are poorly understood. We used next-generation sequencing (NGS) to deep sequence different viruses and strains belonging to this group of flaviviruses, including Central European tick-borne encephalitis virus (TBEV-Eur), Far Eastern TBEV (TBEV-FE), Langat (LGTV), Powassan (POWV), Deer Tick (DTV), Kyasanur Forest Disease (KFDV), Alkhurma hemorrhagic fever (AHFV), and Omsk hemorrhagic fever (OHFV) viruses. DTV, AHFV, and KFDV had the lowest genetic diversity, while POWV strains LEIV-5530 and LB, OHFV, TBEV-Eur, and TBEV-FE had higher genetic diversities. These findings are compatible with the phylogenetic relationships between the viruses. For DTV and POWV, the amount of genetic diversity could be explained by the number of tick vector species and amplification hosts each virus can occupy, with low diversity DTV having a more limited vector and host pool, while POWV with higher genetic diversities has been isolated from different tick species and mammals. It is speculated that high genetic diversity may contribute to the survival of the virus as it encounters these different environments.
AB - The genetic diversities of mammalian tick-borne flaviviruses are poorly understood. We used next-generation sequencing (NGS) to deep sequence different viruses and strains belonging to this group of flaviviruses, including Central European tick-borne encephalitis virus (TBEV-Eur), Far Eastern TBEV (TBEV-FE), Langat (LGTV), Powassan (POWV), Deer Tick (DTV), Kyasanur Forest Disease (KFDV), Alkhurma hemorrhagic fever (AHFV), and Omsk hemorrhagic fever (OHFV) viruses. DTV, AHFV, and KFDV had the lowest genetic diversity, while POWV strains LEIV-5530 and LB, OHFV, TBEV-Eur, and TBEV-FE had higher genetic diversities. These findings are compatible with the phylogenetic relationships between the viruses. For DTV and POWV, the amount of genetic diversity could be explained by the number of tick vector species and amplification hosts each virus can occupy, with low diversity DTV having a more limited vector and host pool, while POWV with higher genetic diversities has been isolated from different tick species and mammals. It is speculated that high genetic diversity may contribute to the survival of the virus as it encounters these different environments.
KW - Alkhurma hemorrhagic fever virus
KW - Deer Tick virus
KW - Far Eastern tick-borne encephalitis virus
KW - Kyasanur Forest Disease virus
KW - Langat virus
KW - Omsk hemorrhagic fever virus
KW - Powassan virus
KW - flavivirus
KW - genetic diversity
KW - tick-borne encephalitis virus
KW - tick-borne viruses
UR - http://www.scopus.com/inward/record.url?scp=85148968435&partnerID=8YFLogxK
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U2 - 10.3390/v15020281
DO - 10.3390/v15020281
M3 - Article
C2 - 36851495
AN - SCOPUS:85148968435
SN - 1999-4915
VL - 15
JO - Viruses
JF - Viruses
IS - 2
M1 - 281
ER -