Differential acute and chronic effects of burn trauma on murine skeletal muscle bioenergetics

Craig Porter, David Herndon, Nisha Bhattarai, John Ogunbileje, Bartosz Szczesny, Csaba Szabo, Tracy Toliver-Kinsky, Labros S. Sidossis

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Altered skeletal muscle mitochondrial function contributes to the pathophysiological stress response to burns. However, the acute and chronic impact of burn trauma on skeletal muscle bioenergetics remains poorly understood. Here, we determined the temporal relationship between burn trauma and mitochondrial function in murine skeletal muscle local to and distal from burn wounds.Male BALB/c mice (8-10 weeks old) were burned by submersion of the dorsum in water (∼95. °C) to create a full thickness burn on ∼30% of the body. Skeletal muscle was harvested spinotrapezius underneath burn wounds (local) and the quadriceps (distal) of sham and burn treated mice at 3. h, 24. h, 4. d and 10. d post-injury. Mitochondrial respiration was determined in permeabilized myofiber bundles by high-resolution respirometry. Caspase 9 and caspase 3 protein concentration were determined by western blot.In muscle local to burn wounds, respiration coupled to ATP production was significantly diminished at 3. h and 24. h post-injury (P

Original languageEnglish (US)
JournalBurns
DOIs
StateAccepted/In press - 2015

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Keywords

  • Apoptosis
  • Burn
  • Hypermetabolism
  • Mitochondria
  • Skeletal muscle

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine
  • Surgery

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