Differential adenoassociated virus vector-driven expression of a neuropeptide Y gene in primary rat brain astroglial cultures after transfection with sendai virosomes versus LipofectinTM

Christopher M. de Fiebre, Ping Wu, Dean Notabartolo, William J. Millard, Edwin M. Meyer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The ability of Sendai virosomes or LipofectinTM to introduce an AAV vector into primary rat brain astroglial cultures was characterized. The pJDT95npy vector was constructed by inserting rat NPY cDNA downstream from the indigenous AAV p5, p19 and p40 promoters in pJDT95. LipofectinTM-mediated transfection with pJDT95npy (10 μg) resulted in pronounced expression of several NPY mRNA species: p5-driven (3.3 kb), p19-driven (2.7 kb) and p40-driven (0.6, 0.8, 1.1, and 1.8 kb). Exposure to virosomally encapsulated pJDT95npy (50 or 100 ng) resulted in transient expression of some p40-driven mRNA species (0.8 and 1.8 kb). Neither method produced astroglia cells which synthesized mature NPY immunoreactivity. This demonstrates that an AAV-derived vector can drive gene expression in astroglia, that Sendai virosomes can infuse vectors into astroglia, but that the amount of DNA infused in this manner may limit long term expression.

Original languageEnglish (US)
Pages (from-to)643-648
Number of pages6
JournalNeurochemical Research
Volume19
Issue number6
DOIs
StatePublished - Jun 1994
Externally publishedYes

Fingerprint

Virosomes
Neuropeptide Y
Viruses
Cell culture
Astrocytes
Transfection
Rats
Brain
Genes
Messenger RNA
Complementary DNA
Gene expression
Gene Expression
DNA
adjuvant P40

Keywords

  • adenoassociated virus vector
  • astroglia
  • Lipofectin
  • neuropeptide Y
  • Virosomes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Differential adenoassociated virus vector-driven expression of a neuropeptide Y gene in primary rat brain astroglial cultures after transfection with sendai virosomes versus LipofectinTM. / de Fiebre, Christopher M.; Wu, Ping; Notabartolo, Dean; Millard, William J.; Meyer, Edwin M.

In: Neurochemical Research, Vol. 19, No. 6, 06.1994, p. 643-648.

Research output: Contribution to journalArticle

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