Abstract
The placental transport and localization in fetal and maternal tissues of 14C-BHA isomers, 2-r-[methy/-14C]butyl-4-hydroxyanisole (2-BHA) and 3-/-[methy/-14Clbutyl-4-hydroxyanisole (3-BHA), were studied in pregnant mice by whole-body autoradiography techniques. BHA isomers were given (iv 50 μCi/100 g as a tracer dose) to pregnant mice at Day 11 (organogenesis) and Day 18 (postorganogenesis) of gestation. Peak levels of radioactivity occurred in various tissues 1-4 hr after iv administration of both isomers. 3-BHA and its metabolites have a higher affinity to fatty tissues and livers of pregnant mice. The concentration of radiocarbon in maternal liver and brown fat following treatment with 14C-3-BHA was much higher than the radioactivity concentration in the corresponding tissues of mothers treated with 2-BHA. On the other hand, the fetal concentration of radioactivity was higher in animals treated with 2-BHA than in those treated with 3-BHA. The radioactivity derived from both isomers accumulated in the fetal gastrointestinal tract. In both groups the radioactivity accumulated in the maternal nasal cavity and mucosa and the gastrointestinal contents. At 24 hr after treatment, retention of radioactivity in maternal lungs, amniotic fluid, and fetal gastrointestinal tissues was observed. Results from this study indicate that there are differences in the magnitude and extent of placental transport of 3-BHA and 2-BHA. Differences also exist in maternal organ uptake and radioactivity distribution of both isomers. Findings from this study are consistent with pharmacological differences existing between the isomers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 356-364 |
| Number of pages | 9 |
| Journal | Toxicological Sciences |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1991 |
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ASJC Scopus subject areas
- Molecular Biology
- Embryology
- Cell Biology
- Genetics
- Developmental Biology
- Toxicology
Cite this
Differential distribution and placental transport of 2- and 3-t-[methy/-c]Butyl-4-hydroxyanisole(BHA) in pregnant mice. / Ahmed, Ahmed E.; Ansari, Ghulam; Lennart, Lennart; Ullberg, Sven.
In: Toxicological Sciences, Vol. 16, No. 2, 02.1991, p. 356-364.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential distribution and placental transport of 2- and 3-t-[methy/-c]Butyl-4-hydroxyanisole(BHA) in pregnant mice
AU - Ahmed, Ahmed E.
AU - Ansari, Ghulam
AU - Lennart, Lennart
AU - Ullberg, Sven
PY - 1991/2
Y1 - 1991/2
N2 - The placental transport and localization in fetal and maternal tissues of 14C-BHA isomers, 2-r-[methy/-14C]butyl-4-hydroxyanisole (2-BHA) and 3-/-[methy/-14Clbutyl-4-hydroxyanisole (3-BHA), were studied in pregnant mice by whole-body autoradiography techniques. BHA isomers were given (iv 50 μCi/100 g as a tracer dose) to pregnant mice at Day 11 (organogenesis) and Day 18 (postorganogenesis) of gestation. Peak levels of radioactivity occurred in various tissues 1-4 hr after iv administration of both isomers. 3-BHA and its metabolites have a higher affinity to fatty tissues and livers of pregnant mice. The concentration of radiocarbon in maternal liver and brown fat following treatment with 14C-3-BHA was much higher than the radioactivity concentration in the corresponding tissues of mothers treated with 2-BHA. On the other hand, the fetal concentration of radioactivity was higher in animals treated with 2-BHA than in those treated with 3-BHA. The radioactivity derived from both isomers accumulated in the fetal gastrointestinal tract. In both groups the radioactivity accumulated in the maternal nasal cavity and mucosa and the gastrointestinal contents. At 24 hr after treatment, retention of radioactivity in maternal lungs, amniotic fluid, and fetal gastrointestinal tissues was observed. Results from this study indicate that there are differences in the magnitude and extent of placental transport of 3-BHA and 2-BHA. Differences also exist in maternal organ uptake and radioactivity distribution of both isomers. Findings from this study are consistent with pharmacological differences existing between the isomers.
AB - The placental transport and localization in fetal and maternal tissues of 14C-BHA isomers, 2-r-[methy/-14C]butyl-4-hydroxyanisole (2-BHA) and 3-/-[methy/-14Clbutyl-4-hydroxyanisole (3-BHA), were studied in pregnant mice by whole-body autoradiography techniques. BHA isomers were given (iv 50 μCi/100 g as a tracer dose) to pregnant mice at Day 11 (organogenesis) and Day 18 (postorganogenesis) of gestation. Peak levels of radioactivity occurred in various tissues 1-4 hr after iv administration of both isomers. 3-BHA and its metabolites have a higher affinity to fatty tissues and livers of pregnant mice. The concentration of radiocarbon in maternal liver and brown fat following treatment with 14C-3-BHA was much higher than the radioactivity concentration in the corresponding tissues of mothers treated with 2-BHA. On the other hand, the fetal concentration of radioactivity was higher in animals treated with 2-BHA than in those treated with 3-BHA. The radioactivity derived from both isomers accumulated in the fetal gastrointestinal tract. In both groups the radioactivity accumulated in the maternal nasal cavity and mucosa and the gastrointestinal contents. At 24 hr after treatment, retention of radioactivity in maternal lungs, amniotic fluid, and fetal gastrointestinal tissues was observed. Results from this study indicate that there are differences in the magnitude and extent of placental transport of 3-BHA and 2-BHA. Differences also exist in maternal organ uptake and radioactivity distribution of both isomers. Findings from this study are consistent with pharmacological differences existing between the isomers.
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UR - http://www.scopus.com/inward/citedby.url?scp=77957179000&partnerID=8YFLogxK
U2 - 10.1093/toxsci/16.2.356
DO - 10.1093/toxsci/16.2.356
M3 - Article
C2 - 2055366
AN - SCOPUS:77957179000
VL - 16
SP - 356
EP - 364
JO - Toxicological Sciences
JF - Toxicological Sciences
SN - 1096-6080
IS - 2
ER -