TY - JOUR
T1 - Differential effects of monoclonal antibody blockade of adhesion molecules on in vivo susceptibility to soft tissue infection
AU - Garcia, N.
AU - Mileski, W. J.
AU - Lipsky, P.
PY - 1995
Y1 - 1995
N2 - Leukocyte adherence to endothelial cells has been implicated in the pathogenesis of microvascular injury as well as in host defense against various infectious microorganisms. Administration of monoclonal antibodies directed against the β chain of the leukocyte integrins inhibits leukocyte- endothelial-cell adherence and has been reported to modulate ischemia- reperfusion and inflammatory injury. However, such inhibition of adhesion molecule function adversely affects resistance to infection. The following studies were carried out to determine whether monoclonal antibodies to other adhesion molecules, including L-selectin (CD62L), and CD11a (the α chain of LFA-1), also increase susceptibility to infection. New Zealand White rabbits were shaved and given subcutaneous injections on their dorsa with 109 CFU of Staphylococcus aureus ATCC 25923 at two sites and with 108 CFU at two sites. A second set of rabbits were given subcutaneous injections with 108 CFU of P. aeruginosa ATCC 27853 at two sites and with 107 CFUs at two sites. The animals were monitored for 1 week. There were three blinded experimental groups: controls given saline and two groups given blocking monoclonal antibodies to either L-selectin (Dreg-200) or CD11a (R7.1). In contrast to monoclonal antibodies to CD18, none of the monoclonal antibodies significantly increased the risk of abscess formation by S. aureus, although inhibition of CD11a increased the rate of abscess formation by P. aeruginosa.
AB - Leukocyte adherence to endothelial cells has been implicated in the pathogenesis of microvascular injury as well as in host defense against various infectious microorganisms. Administration of monoclonal antibodies directed against the β chain of the leukocyte integrins inhibits leukocyte- endothelial-cell adherence and has been reported to modulate ischemia- reperfusion and inflammatory injury. However, such inhibition of adhesion molecule function adversely affects resistance to infection. The following studies were carried out to determine whether monoclonal antibodies to other adhesion molecules, including L-selectin (CD62L), and CD11a (the α chain of LFA-1), also increase susceptibility to infection. New Zealand White rabbits were shaved and given subcutaneous injections on their dorsa with 109 CFU of Staphylococcus aureus ATCC 25923 at two sites and with 108 CFU at two sites. A second set of rabbits were given subcutaneous injections with 108 CFU of P. aeruginosa ATCC 27853 at two sites and with 107 CFUs at two sites. The animals were monitored for 1 week. There were three blinded experimental groups: controls given saline and two groups given blocking monoclonal antibodies to either L-selectin (Dreg-200) or CD11a (R7.1). In contrast to monoclonal antibodies to CD18, none of the monoclonal antibodies significantly increased the risk of abscess formation by S. aureus, although inhibition of CD11a increased the rate of abscess formation by P. aeruginosa.
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U2 - 10.1128/iai.63.10.3816-3819.1995
DO - 10.1128/iai.63.10.3816-3819.1995
M3 - Article
C2 - 7558285
AN - SCOPUS:0029093918
SN - 0019-9567
VL - 63
SP - 3816
EP - 3819
JO - Infection and immunity
JF - Infection and immunity
IS - 10
ER -