Differential effects of p-chlorophenylalanine on indoleamines in brainstem nuclei and spinal cord of rats. II. Identification of immunohistochemically stained structures using computer-assisted image enhancement techniques

Susan M. Carlton, Judith L. Steinman, Gilbert R. Hillman, William D. Willis

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Following intraperitoneal injection of p-chlorophenylalanine (PCPA, 400 and 600 mg/kg) on 3 consecutive days, the brainstem and lumbar cord of rats were removed, frozen-sectioned and immunohistochemically stained (PAP method) for serotonin (5-HT). Using computer-assisted image analysis, the density of 5-HT staining in control, 400 and 600 mg/kg PCPA groups was determined. The mean number of pixels (representing 5-HT staining) was determined in 6 areas in the brainstem containing 5-HT cell bodies (nuclei raphe pallidus, raphe obscurus, rostral and caudal raphe magnus, raphe dorsalis and paragigantocellularis lateralis) and in the dorsal and ventral spinal cord. The resultssuggest a differential depletion of 5-HT within brainstem nuclei following PCPA treatment in that the most marked dose-related reductions were observed in nucleus raphe obscurus and caudal magnus. Furthermore, a computer program designed to isolate terminal structures in the spinal cord identified a differential depletion of 5-HT terminals in the dorsal horn versus the ventral horn. The present study describes 3 analytical approaches combining immunohistochemistry with the computer-assisted image analysis technique and allows comparison between groups of animals which received the same or different drug treatments.

Original languageEnglish (US)
Pages (from-to)310-322
Number of pages13
JournalBrain Research
Volume426
Issue number2
DOIs
StatePublished - Nov 24 1987

Fingerprint

Image Enhancement
Fenclonine
Brain Stem
Serotonin
Spinal Cord
Computer-Assisted Image Processing
Staining and Labeling
Horns
Intraperitoneal Injections
Cell Nucleus
Software
Immunohistochemistry
Therapeutics

Keywords

  • Dorsal spinal cord
  • Image analysis
  • Raphe nucleus
  • Serotonin
  • Ventral spinals cord

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Differential effects of p-chlorophenylalanine on indoleamines in brainstem nuclei and spinal cord of rats. II. Identification of immunohistochemically stained structures using computer-assisted image enhancement techniques. / Carlton, Susan M.; Steinman, Judith L.; Hillman, Gilbert R.; Willis, William D.

In: Brain Research, Vol. 426, No. 2, 24.11.1987, p. 310-322.

Research output: Contribution to journalArticle

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