Differential effects of respiratory syncytial virus and adenovirus on mononuclear ceil cytokine responses

Patricia V. Díaz, William Calhoun, Kim L. Hinton, Luis F. Avendaño, Aldo Gaggero, Valeska Simon, Sonia M. Arredondo, Ricardo Pinto, Armando Díaz

Research output: Contribution to journalArticle

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Abstract

Respiratory syncytial virus (RSV) and adenovirus (Advs) serotype 3 (Adv3) and 7h (Adv7h) are associated with mild to severe respiratory infection and are indistinguishable during the acute phases of the illnesses. However, outcome and long-term prognosis are different with both infections. RSV infection is associated with later development of asthma, and Adv, mainly Adv7h, with severe lung damage, bronchiectasis, and hyperlucent lung. We hypothesized that this difference could be partly due to different immune responses induced by these viruses. To test this hypothesis we quantified TCD4+, TCD8+, and BCD19+ expressing the interleukin-2 receptor-alpha chain (CD25) and interferon-γ (IFN-γ), interleukin (IL)-10, and IL-4 in the supernatant of peripheral blood mononuclear cells (PBMC) from school children infected in vitro with and without RSV, Adv7h, and Adv3 and after phytohemagglutinin (PHA) stimulation in the presence or absence of these viruses at a multiplicity of infection (MOI) of 1. PBMC from every child produced more IL-10 (p ≤ 0.05) when infected with RSV than with Advs and noninfected control, and Adv induced more (p ≤ 0.05) IFN-γ than did RSV and control. The IL-10/IFN-γ ratio was significantly higher (p ≤ 0.05) in RSV- infected and significantly lower (p ≤ 0.05) in Adv-infected PBMC, than in noninfected cells. PHA-stimulated BCD19+ RSV-infected cells expressed more (p ≤ 0.05) IL-2R than did Adv-infected cells. These results suggest that Advs induce a Th-1-type immune response that is not seen with RSV. These patterns persist despite intersubject variation in the absolute quantity of cytokine produced.

Original languageEnglish (US)
Pages (from-to)1157-1164
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume160
Issue number4
StatePublished - 1999
Externally publishedYes

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Respiratory Syncytial Viruses
Adenoviridae
Cytokines
Interleukin-10
Blood Cells
Phytohemagglutinins
Interferons
Hyperlucent Lung
Interleukin-2 Receptor alpha Subunit
Viruses
Respiratory Syncytial Virus Infections
Bronchiectasis
Interleukins
Infection
Interferon-alpha
Respiratory Tract Infections
Interleukin-4
Asthma
Lung

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Differential effects of respiratory syncytial virus and adenovirus on mononuclear ceil cytokine responses. / Díaz, Patricia V.; Calhoun, William; Hinton, Kim L.; Avendaño, Luis F.; Gaggero, Aldo; Simon, Valeska; Arredondo, Sonia M.; Pinto, Ricardo; Díaz, Armando.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 160, No. 4, 1999, p. 1157-1164.

Research output: Contribution to journalArticle

Díaz, PV, Calhoun, W, Hinton, KL, Avendaño, LF, Gaggero, A, Simon, V, Arredondo, SM, Pinto, R & Díaz, A 1999, 'Differential effects of respiratory syncytial virus and adenovirus on mononuclear ceil cytokine responses', American Journal of Respiratory and Critical Care Medicine, vol. 160, no. 4, pp. 1157-1164.
Díaz, Patricia V. ; Calhoun, William ; Hinton, Kim L. ; Avendaño, Luis F. ; Gaggero, Aldo ; Simon, Valeska ; Arredondo, Sonia M. ; Pinto, Ricardo ; Díaz, Armando. / Differential effects of respiratory syncytial virus and adenovirus on mononuclear ceil cytokine responses. In: American Journal of Respiratory and Critical Care Medicine. 1999 ; Vol. 160, No. 4. pp. 1157-1164.
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