Differential effects of sodium butyrate and hexamethylene bisacetamide on growth and secretion of cultured human endocrine tumor cells

D. Parekh, J. Ishizuka, Courtney Townsend, B. E. Haber, R. D. Beauchamp, S. Rajaraman, G. Karp, J. Hsieh, J. C. Thompson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Advanced gastrointestinal endocrine tumors respond poorly to conventional chemotherapy. In this study we examined the effects of two agents that promote cellular differention, sodium butyrate and hexamethylene bisacetamide, on the in vitro growth and secretory responses of a human pancreatic carcinoid (BON) and human gastrinoma (PT-2 and PT-SM) cell lines that have been established in our laboratory. We found that both sodium butyrate and hexamethylene bisacetamide strongly inhibited growth of BON, PT-2, and PT-SM cells. With continuous exposure of BON cells to sodium butyrate (2 mmol/L), the doubling time was prolonged, from 60 hours in controls to 156 hours, and saturation density was reduced to 28% that of controls. Hexamethylene bisacetamide (4 mmol/L) reduced saturation density to 37% that of controls in BON cells and prolonged the doubling time, from 60 hours to 103 hours. Antiproliferative effects of similar magnitudes were observed in the gastrinoma cell lines. In contrast, differential effects were produced on amine biosynthesis in BON cells; sodium butyrate stimulated levels of 5-hydroxytryptamine in the cells, whereas hexamethylene bisacetamide caused a profound dose-dependent inhibition of amine biosynthesis. The significant antiproliferative activity of sodium butyrate and hexamethylene bisacetamide and the inhibitory effects of hexamethylene bisacetamide on amine biosynthesis warrant evaluation of these agents or analogues for treatment of metastatic carcinoid and gastrinoma.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalArchives of Surgery
Volume126
Issue number4
StatePublished - 1991

Fingerprint

hexamethylene bisacetamide
Endocrine Cells
Butyric Acid
Gastrinoma
Growth
Amines
Neoplasms
Carcinoid Tumor
Cell Line
Serotonin

ASJC Scopus subject areas

  • Surgery

Cite this

Parekh, D., Ishizuka, J., Townsend, C., Haber, B. E., Beauchamp, R. D., Rajaraman, S., ... Thompson, J. C. (1991). Differential effects of sodium butyrate and hexamethylene bisacetamide on growth and secretion of cultured human endocrine tumor cells. Archives of Surgery, 126(4), 467-472.

Differential effects of sodium butyrate and hexamethylene bisacetamide on growth and secretion of cultured human endocrine tumor cells. / Parekh, D.; Ishizuka, J.; Townsend, Courtney; Haber, B. E.; Beauchamp, R. D.; Rajaraman, S.; Karp, G.; Hsieh, J.; Thompson, J. C.

In: Archives of Surgery, Vol. 126, No. 4, 1991, p. 467-472.

Research output: Contribution to journalArticle

Parekh, D, Ishizuka, J, Townsend, C, Haber, BE, Beauchamp, RD, Rajaraman, S, Karp, G, Hsieh, J & Thompson, JC 1991, 'Differential effects of sodium butyrate and hexamethylene bisacetamide on growth and secretion of cultured human endocrine tumor cells', Archives of Surgery, vol. 126, no. 4, pp. 467-472.
Parekh, D. ; Ishizuka, J. ; Townsend, Courtney ; Haber, B. E. ; Beauchamp, R. D. ; Rajaraman, S. ; Karp, G. ; Hsieh, J. ; Thompson, J. C. / Differential effects of sodium butyrate and hexamethylene bisacetamide on growth and secretion of cultured human endocrine tumor cells. In: Archives of Surgery. 1991 ; Vol. 126, No. 4. pp. 467-472.
@article{d2609466899f4a01bae2694379c5ff35,
title = "Differential effects of sodium butyrate and hexamethylene bisacetamide on growth and secretion of cultured human endocrine tumor cells",
abstract = "Advanced gastrointestinal endocrine tumors respond poorly to conventional chemotherapy. In this study we examined the effects of two agents that promote cellular differention, sodium butyrate and hexamethylene bisacetamide, on the in vitro growth and secretory responses of a human pancreatic carcinoid (BON) and human gastrinoma (PT-2 and PT-SM) cell lines that have been established in our laboratory. We found that both sodium butyrate and hexamethylene bisacetamide strongly inhibited growth of BON, PT-2, and PT-SM cells. With continuous exposure of BON cells to sodium butyrate (2 mmol/L), the doubling time was prolonged, from 60 hours in controls to 156 hours, and saturation density was reduced to 28{\%} that of controls. Hexamethylene bisacetamide (4 mmol/L) reduced saturation density to 37{\%} that of controls in BON cells and prolonged the doubling time, from 60 hours to 103 hours. Antiproliferative effects of similar magnitudes were observed in the gastrinoma cell lines. In contrast, differential effects were produced on amine biosynthesis in BON cells; sodium butyrate stimulated levels of 5-hydroxytryptamine in the cells, whereas hexamethylene bisacetamide caused a profound dose-dependent inhibition of amine biosynthesis. The significant antiproliferative activity of sodium butyrate and hexamethylene bisacetamide and the inhibitory effects of hexamethylene bisacetamide on amine biosynthesis warrant evaluation of these agents or analogues for treatment of metastatic carcinoid and gastrinoma.",
author = "D. Parekh and J. Ishizuka and Courtney Townsend and Haber, {B. E.} and Beauchamp, {R. D.} and S. Rajaraman and G. Karp and J. Hsieh and Thompson, {J. C.}",
year = "1991",
language = "English",
volume = "126",
pages = "467--472",
journal = "JAMA Surgery",
issn = "2168-6254",
publisher = "American Medical Association",
number = "4",

}

TY - JOUR

T1 - Differential effects of sodium butyrate and hexamethylene bisacetamide on growth and secretion of cultured human endocrine tumor cells

AU - Parekh, D.

AU - Ishizuka, J.

AU - Townsend, Courtney

AU - Haber, B. E.

AU - Beauchamp, R. D.

AU - Rajaraman, S.

AU - Karp, G.

AU - Hsieh, J.

AU - Thompson, J. C.

PY - 1991

Y1 - 1991

N2 - Advanced gastrointestinal endocrine tumors respond poorly to conventional chemotherapy. In this study we examined the effects of two agents that promote cellular differention, sodium butyrate and hexamethylene bisacetamide, on the in vitro growth and secretory responses of a human pancreatic carcinoid (BON) and human gastrinoma (PT-2 and PT-SM) cell lines that have been established in our laboratory. We found that both sodium butyrate and hexamethylene bisacetamide strongly inhibited growth of BON, PT-2, and PT-SM cells. With continuous exposure of BON cells to sodium butyrate (2 mmol/L), the doubling time was prolonged, from 60 hours in controls to 156 hours, and saturation density was reduced to 28% that of controls. Hexamethylene bisacetamide (4 mmol/L) reduced saturation density to 37% that of controls in BON cells and prolonged the doubling time, from 60 hours to 103 hours. Antiproliferative effects of similar magnitudes were observed in the gastrinoma cell lines. In contrast, differential effects were produced on amine biosynthesis in BON cells; sodium butyrate stimulated levels of 5-hydroxytryptamine in the cells, whereas hexamethylene bisacetamide caused a profound dose-dependent inhibition of amine biosynthesis. The significant antiproliferative activity of sodium butyrate and hexamethylene bisacetamide and the inhibitory effects of hexamethylene bisacetamide on amine biosynthesis warrant evaluation of these agents or analogues for treatment of metastatic carcinoid and gastrinoma.

AB - Advanced gastrointestinal endocrine tumors respond poorly to conventional chemotherapy. In this study we examined the effects of two agents that promote cellular differention, sodium butyrate and hexamethylene bisacetamide, on the in vitro growth and secretory responses of a human pancreatic carcinoid (BON) and human gastrinoma (PT-2 and PT-SM) cell lines that have been established in our laboratory. We found that both sodium butyrate and hexamethylene bisacetamide strongly inhibited growth of BON, PT-2, and PT-SM cells. With continuous exposure of BON cells to sodium butyrate (2 mmol/L), the doubling time was prolonged, from 60 hours in controls to 156 hours, and saturation density was reduced to 28% that of controls. Hexamethylene bisacetamide (4 mmol/L) reduced saturation density to 37% that of controls in BON cells and prolonged the doubling time, from 60 hours to 103 hours. Antiproliferative effects of similar magnitudes were observed in the gastrinoma cell lines. In contrast, differential effects were produced on amine biosynthesis in BON cells; sodium butyrate stimulated levels of 5-hydroxytryptamine in the cells, whereas hexamethylene bisacetamide caused a profound dose-dependent inhibition of amine biosynthesis. The significant antiproliferative activity of sodium butyrate and hexamethylene bisacetamide and the inhibitory effects of hexamethylene bisacetamide on amine biosynthesis warrant evaluation of these agents or analogues for treatment of metastatic carcinoid and gastrinoma.

UR - http://www.scopus.com/inward/record.url?scp=0025877042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025877042&partnerID=8YFLogxK

M3 - Article

VL - 126

SP - 467

EP - 472

JO - JAMA Surgery

JF - JAMA Surgery

SN - 2168-6254

IS - 4

ER -