Differential expression and regulation of thyrotropin (TSH) in T cell lines

Deborah V. Harbour, Thomas E. Kruger, Dorian Coppenhaver, Eric M. Smith, Walter J. Meyer

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The immune system's production of and response to thyrotropin (TSH) suggests the existence of a hypothalamic-lymphoid-thyroid regulatory axis (HLT). To evaluate the possible roles of lymphocyte-derived TSH we characterized its structure and regulation of production. Using an in vitro system, we screened T cell lines for the production of TSH in response to thyrotropin-releasing hormone (TRH). We identified MOLT 4 T cells as producers of intact TSH based on TSH radioimmunoassay (RIA) displacement and de novo synthesized protein structure similar to pituitary TSH. Northern blot analysis using a cDNA probe to TSHβ showed the existence of an mRNA species similar in molecular size to pituitary mRNA of TSHβ. The MOLT 4-derived TSH is induced in a dose-dependent fashion by TRH and that induction is significantly inhibited by the thyroid hormone, triiodothyronine (T3). HUT 78 T cells express an mRNA species similar to TSHβ but do not express detectable protein suggesting a lack of translation of the mRNA species. The evidence suggests that the MOLT 4 T cells serve as an in vitro model for TSH production and may be used to study differential expression of the genes for TSH in the immune system. Elucidation of this in vitro system will provide information on mechanisms of regulation of an HLT axis that are unique to the immune system as well as pathways common to the immune and neuroendocrine systems.

Original languageEnglish (US)
Pages (from-to)229-241
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume64
Issue number2
DOIs
StatePublished - Jul 1989

Keywords

  • Lymphocyte-derived thyrotropin
  • Thyrotropin gene expression
  • Thyrotropin regulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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    Harbour, D. V., Kruger, T. E., Coppenhaver, D., Smith, E. M., & Meyer, W. J. (1989). Differential expression and regulation of thyrotropin (TSH) in T cell lines. Molecular and Cellular Endocrinology, 64(2), 229-241. https://doi.org/10.1016/0303-7207(89)90150-0