Differential expression of alpha1-adrenoceptor subtype mRNAs in the dorsal root ganglion after spinal nerve ligation

Jiangang Xie, Young Ho Lee, Chen Wang, Jin Chung, Kyungsoon Chung

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

In spinal nerve ligated Lewis strain neuropathic rats, pain behaviors and the rate of ectopic discharges of injured sensory neurons were significantly reduced by systemic injection of phentolamine. A pharmacological study indicated that this adrenergic dependency was mediated by α1-adrenoceptors (α1-AR). The development of adrenergic sensitivity in injured sensory neurons might have resulted from changes in adrenoceptor expression as a consequence of changed expression of adrenoceptor genes. This possibility was examined by determining the changes in the mRNA expression of 3 subtypes of α1-ARs, α1a-, α1b-, and α1d-ARs, in the dorsal root ganglia (DRG) after spinal nerve ligation. The L4 and L5 spinal nerves were tightly ligated in Lewis rats. One week later, the L4 and L5 DRG were collected and RNase protection assay (RPA) and in situ hybridization were performed. In the DRG of unoperated rats, a moderate amount of α1a-AR mRNA was present while the amount of either α1b-AR or α1d-AR mRNA was small. After spinal nerve ligation, there was a significant increase in the amount of α1b-AR mRNA in the nerve ligated DRG as measured by RPA. The amount of α1a-AR mRNA was decreased to 20% of the normal level while that of α1d-AR mRNA did not change. The in situ hybridization study showed that the number of α1b-AR mRNA positive neurons increased in spinal nerve ligated DRG, confirming the results of RPA study. These data suggest that the up-regulated expression of α1b-AR mRNA in axotomized DRG neurons may play an important role in the development of adrenergic sensitivity in injured sensory neurons and thus contribute to the sympathetically maintained pain in spinal nerve ligated neuropathic Lewis rats.

Original languageEnglish (US)
Pages (from-to)164-172
Number of pages9
JournalMolecular Brain Research
Volume93
Issue number2
DOIs
StatePublished - Sep 30 2001

Fingerprint

Spinal Nerves
Spinal Ganglia
Adrenergic Receptors
Ligation
Messenger RNA
Sensory Receptor Cells
Ribonucleases
Adrenergic Agents
In Situ Hybridization
Neurons
Phentolamine
Neuralgia
Pharmacology
Gene Expression
Pain
Injections

Keywords

  • Axotomized sensory neuron
  • In situ hybridization
  • Lewis strain of rats
  • Neuropathic pain
  • RNase protection assay
  • Sympathetically maintained pain

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Differential expression of alpha1-adrenoceptor subtype mRNAs in the dorsal root ganglion after spinal nerve ligation. / Xie, Jiangang; Ho Lee, Young; Wang, Chen; Chung, Jin; Chung, Kyungsoon.

In: Molecular Brain Research, Vol. 93, No. 2, 30.09.2001, p. 164-172.

Research output: Contribution to journalArticle

Xie, Jiangang ; Ho Lee, Young ; Wang, Chen ; Chung, Jin ; Chung, Kyungsoon. / Differential expression of alpha1-adrenoceptor subtype mRNAs in the dorsal root ganglion after spinal nerve ligation. In: Molecular Brain Research. 2001 ; Vol. 93, No. 2. pp. 164-172.
@article{4a4bda30b89b4a8bb5e9063ffb1e52e1,
title = "Differential expression of alpha1-adrenoceptor subtype mRNAs in the dorsal root ganglion after spinal nerve ligation",
abstract = "In spinal nerve ligated Lewis strain neuropathic rats, pain behaviors and the rate of ectopic discharges of injured sensory neurons were significantly reduced by systemic injection of phentolamine. A pharmacological study indicated that this adrenergic dependency was mediated by α1-adrenoceptors (α1-AR). The development of adrenergic sensitivity in injured sensory neurons might have resulted from changes in adrenoceptor expression as a consequence of changed expression of adrenoceptor genes. This possibility was examined by determining the changes in the mRNA expression of 3 subtypes of α1-ARs, α1a-, α1b-, and α1d-ARs, in the dorsal root ganglia (DRG) after spinal nerve ligation. The L4 and L5 spinal nerves were tightly ligated in Lewis rats. One week later, the L4 and L5 DRG were collected and RNase protection assay (RPA) and in situ hybridization were performed. In the DRG of unoperated rats, a moderate amount of α1a-AR mRNA was present while the amount of either α1b-AR or α1d-AR mRNA was small. After spinal nerve ligation, there was a significant increase in the amount of α1b-AR mRNA in the nerve ligated DRG as measured by RPA. The amount of α1a-AR mRNA was decreased to 20{\%} of the normal level while that of α1d-AR mRNA did not change. The in situ hybridization study showed that the number of α1b-AR mRNA positive neurons increased in spinal nerve ligated DRG, confirming the results of RPA study. These data suggest that the up-regulated expression of α1b-AR mRNA in axotomized DRG neurons may play an important role in the development of adrenergic sensitivity in injured sensory neurons and thus contribute to the sympathetically maintained pain in spinal nerve ligated neuropathic Lewis rats.",
keywords = "Axotomized sensory neuron, In situ hybridization, Lewis strain of rats, Neuropathic pain, RNase protection assay, Sympathetically maintained pain",
author = "Jiangang Xie and {Ho Lee}, Young and Chen Wang and Jin Chung and Kyungsoon Chung",
year = "2001",
month = "9",
day = "30",
doi = "10.1016/S0169-328X(01)00201-7",
language = "English (US)",
volume = "93",
pages = "164--172",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Differential expression of alpha1-adrenoceptor subtype mRNAs in the dorsal root ganglion after spinal nerve ligation

AU - Xie, Jiangang

AU - Ho Lee, Young

AU - Wang, Chen

AU - Chung, Jin

AU - Chung, Kyungsoon

PY - 2001/9/30

Y1 - 2001/9/30

N2 - In spinal nerve ligated Lewis strain neuropathic rats, pain behaviors and the rate of ectopic discharges of injured sensory neurons were significantly reduced by systemic injection of phentolamine. A pharmacological study indicated that this adrenergic dependency was mediated by α1-adrenoceptors (α1-AR). The development of adrenergic sensitivity in injured sensory neurons might have resulted from changes in adrenoceptor expression as a consequence of changed expression of adrenoceptor genes. This possibility was examined by determining the changes in the mRNA expression of 3 subtypes of α1-ARs, α1a-, α1b-, and α1d-ARs, in the dorsal root ganglia (DRG) after spinal nerve ligation. The L4 and L5 spinal nerves were tightly ligated in Lewis rats. One week later, the L4 and L5 DRG were collected and RNase protection assay (RPA) and in situ hybridization were performed. In the DRG of unoperated rats, a moderate amount of α1a-AR mRNA was present while the amount of either α1b-AR or α1d-AR mRNA was small. After spinal nerve ligation, there was a significant increase in the amount of α1b-AR mRNA in the nerve ligated DRG as measured by RPA. The amount of α1a-AR mRNA was decreased to 20% of the normal level while that of α1d-AR mRNA did not change. The in situ hybridization study showed that the number of α1b-AR mRNA positive neurons increased in spinal nerve ligated DRG, confirming the results of RPA study. These data suggest that the up-regulated expression of α1b-AR mRNA in axotomized DRG neurons may play an important role in the development of adrenergic sensitivity in injured sensory neurons and thus contribute to the sympathetically maintained pain in spinal nerve ligated neuropathic Lewis rats.

AB - In spinal nerve ligated Lewis strain neuropathic rats, pain behaviors and the rate of ectopic discharges of injured sensory neurons were significantly reduced by systemic injection of phentolamine. A pharmacological study indicated that this adrenergic dependency was mediated by α1-adrenoceptors (α1-AR). The development of adrenergic sensitivity in injured sensory neurons might have resulted from changes in adrenoceptor expression as a consequence of changed expression of adrenoceptor genes. This possibility was examined by determining the changes in the mRNA expression of 3 subtypes of α1-ARs, α1a-, α1b-, and α1d-ARs, in the dorsal root ganglia (DRG) after spinal nerve ligation. The L4 and L5 spinal nerves were tightly ligated in Lewis rats. One week later, the L4 and L5 DRG were collected and RNase protection assay (RPA) and in situ hybridization were performed. In the DRG of unoperated rats, a moderate amount of α1a-AR mRNA was present while the amount of either α1b-AR or α1d-AR mRNA was small. After spinal nerve ligation, there was a significant increase in the amount of α1b-AR mRNA in the nerve ligated DRG as measured by RPA. The amount of α1a-AR mRNA was decreased to 20% of the normal level while that of α1d-AR mRNA did not change. The in situ hybridization study showed that the number of α1b-AR mRNA positive neurons increased in spinal nerve ligated DRG, confirming the results of RPA study. These data suggest that the up-regulated expression of α1b-AR mRNA in axotomized DRG neurons may play an important role in the development of adrenergic sensitivity in injured sensory neurons and thus contribute to the sympathetically maintained pain in spinal nerve ligated neuropathic Lewis rats.

KW - Axotomized sensory neuron

KW - In situ hybridization

KW - Lewis strain of rats

KW - Neuropathic pain

KW - RNase protection assay

KW - Sympathetically maintained pain

UR - http://www.scopus.com/inward/record.url?scp=0035975650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035975650&partnerID=8YFLogxK

U2 - 10.1016/S0169-328X(01)00201-7

DO - 10.1016/S0169-328X(01)00201-7

M3 - Article

VL - 93

SP - 164

EP - 172

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -